TY - JOUR
T1 - Yogurt Benefits Bone Mineralization in Ovariectomized Rats with Concomitant Modulation of the Gut Microbiome
AU - He, Weiwei
AU - Xie, Zhuqing
AU - Wittig, Nina Kølln
AU - Zachariassen, Line F
AU - Andersen, Amanda
AU - Andersen, Henrik J
AU - Birkedal, Henrik
AU - Nielsen, Dennis S
AU - Kornerup Hansen, Axel
AU - Bertram, Hanne Christine
PY - 2022/10
Y1 - 2022/10
N2 - SCOPE: Evidence supports that gut-modulating foods potentially can supress bone loss in postmenopausal women. This study aimed to investigate the effect of milk calcium-enriched milk, yogurt, and yogurt-inulin combination on the gut-bone association.METHODS AND RESULTS: A 6-week intervention study was conducted in ovariectomized rats. Four pastes containing milk calcium-fortified milk (M-Ca), milk calcium-fortified yogurt (Y-Ca), inulin-fortified Y-Ca (Y-I-Ca), or an isoconcentration of calcium carbonate (Ca-N), and a calcium-deficient paste were provided. M-Ca did not influence bone mineral density and content (BMD and BMC), femur mechanical strength, or femoral microstructure compared to Ca-N, but Y-Ca increased spine BMD. The serum metabolome revealed that Y-Ca modulated glycine-related pathways with reduced glycine, serine and threonine. No additive effects of yogurt and inulin were found on bone parameters. Correlation analysis showed that increased lactobacilli and reduced Clostridiaceae members in Y-Ca was associated with an increased spine BMD. Increases in Bifidobacterium pseudolongum, Turicibacter, Blautia, and Allobaculum and gut short-chain fatty acids in Y-I-Ca were not reflected in bone parameters.CONCLUSION: Yogurt as calcium vehicle contributed to increased spine BMD concomitant with changes in the gut microbiome and glycine-related pathways, while adding inulin to yogurt did not affect bone mineralization in ovariectomized rats. This article is protected by copyright. All rights reserved.
AB - SCOPE: Evidence supports that gut-modulating foods potentially can supress bone loss in postmenopausal women. This study aimed to investigate the effect of milk calcium-enriched milk, yogurt, and yogurt-inulin combination on the gut-bone association.METHODS AND RESULTS: A 6-week intervention study was conducted in ovariectomized rats. Four pastes containing milk calcium-fortified milk (M-Ca), milk calcium-fortified yogurt (Y-Ca), inulin-fortified Y-Ca (Y-I-Ca), or an isoconcentration of calcium carbonate (Ca-N), and a calcium-deficient paste were provided. M-Ca did not influence bone mineral density and content (BMD and BMC), femur mechanical strength, or femoral microstructure compared to Ca-N, but Y-Ca increased spine BMD. The serum metabolome revealed that Y-Ca modulated glycine-related pathways with reduced glycine, serine and threonine. No additive effects of yogurt and inulin were found on bone parameters. Correlation analysis showed that increased lactobacilli and reduced Clostridiaceae members in Y-Ca was associated with an increased spine BMD. Increases in Bifidobacterium pseudolongum, Turicibacter, Blautia, and Allobaculum and gut short-chain fatty acids in Y-I-Ca were not reflected in bone parameters.CONCLUSION: Yogurt as calcium vehicle contributed to increased spine BMD concomitant with changes in the gut microbiome and glycine-related pathways, while adding inulin to yogurt did not affect bone mineralization in ovariectomized rats. This article is protected by copyright. All rights reserved.
KW - NMR metabolomics
KW - fermented dairy
KW - gut metabolome
KW - gut–bone axis
KW - inulin
UR - http://www.scopus.com/inward/record.url?scp=85138008534&partnerID=8YFLogxK
U2 - 10.1002/mnfr.202200174
DO - 10.1002/mnfr.202200174
M3 - Journal article
C2 - 36039478
SN - 1613-4125
VL - 66
JO - Molecular Nutrition & Food Research
JF - Molecular Nutrition & Food Research
IS - 20
M1 - 2200174
ER -