Whole-genome sequencing of aggregatibacter species isolated from human clinical specimens and description of Aggregatibacter kilianii sp. Nov.

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  • May Murra
  • ,
  • Lisbeth Lützen, Aalborg University
  • ,
  • Aynur Barut
  • ,
  • Reinhard Zbinden, University of Zurich
  • ,
  • Marianne Lund
  • ,
  • Palle Villesen
  • Niels Nørskov-Lauritsen

Aggregatibacter species are commensal bacteria of human mucosal surfaces that are sometimes involved in serious invasive infections. During the investigation of strains cultured from various clinical specimens, we encountered a coherent group of 10 isolates that could not be allocated to any validly named species by phenotype, mass spectrometry, or partial 16S rRNA gene sequencing. Whole-genome sequencing revealed a phylogenetic cluster related to but separate from Aggregatibacter aphrophilus. The mean in silico DNA hybridization value for strains of the new cluster versus A. Aphrophilus was 56% (range, 53.7 to 58.0%), whereas the average nucleotide identity was 94.4% (range, 93.9 to 94.8%). The new cluster exhibited aggregative properties typical of the genus Aggregatibacter. Key phenotypic tests for discrimination of the new cluster from validly named Aggregatibacter species are alanine-phenylalanine-proline arylamidase, N-acetylglucosamine, and-galactosidase. The name Aggregatibacter kilianii is proposed, with PN528 (CCUG 70536T or DSM 105094T) as the type strain.

Original languageEnglish
Article numberUNSP e00053-18
JournalJournal of Clinical Microbiology
Number of pages10
Publication statusPublished - Jul 2018

    Research areas

  • Abscesses, Average nucleotide identity, HACEK, Haemophilus, Phylogeny

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