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Whole‐Genome Sequence of Synthesized Allopolyploids in Cucumis Reveals Insights into the Genome Evolution of Allopolyploidization

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DOI

  • Xiaqing Yu, Nanjing Agricultural University
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  • Panqiao Wang, Nanjing Agricultural University
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  • Ji Li, Nanjing Agricultural University
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  • Qinzheng Zhao, Nanjing Agricultural University
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  • Changmian Ji, Chinese Academy of Tropical Agricultural Sciences
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  • Zaobing Zhu, Nanjing Agricultural University
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  • Yufei Zhai, Nanjing Agricultural University
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  • Xiaodong Qin, Nanjing Agricultural University
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  • Junguo Zhou, Henan Institute of Science and Technology
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  • Haiyan Yu, Biomarker Technologies
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  • Xinchao Cheng, Biomarker Technologies
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  • Shiro Isshiki, Saga University
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  • Molly Jahn, Jahn Research Group, USDA/FPL
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  • Jeff J. Doyle, Cornell University
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  • Carl-Otto Ottosen
  • Yuling Bai, Wageningen University and Research
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  • Qinsheng Cai, Nanjing Agricultural University
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  • Chunyan Cheng, Nanjing Agricultural University
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  • Qunfeng Lou, Nanjing Agricultural University
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  • Sanwen Huang, Chinese Academy of Agricultural Sciences, Denmark
  • Jinfeng Chen, Nanjing Agricultural University, Denmark
The importance of allopolyploidy in plant evolution has been widely recognized. The genetic changes triggered by allopolyploidy, however, are not yet fully understood due to inconsistent phenomena reported across diverse species. The construction of synthetic polyploids offers a controlled approach to systematically reveal genomic changes that occur during the process of polyploidy. This study reports the first fully sequenced synthetic allopolyploid constructed from a cross between Cucumis sativus and C. hystrix, with high‐quality assembly. The two subgenomes are confidently partitioned and the C. sativus‐originated subgenome predominates over the C. hystrix‐originated subgenome, retaining more sequences and showing higher homeologous gene expression. Most of the genomic changes emerge immediately after interspecific hybridization. Analysis of a series of genome sequences from several generations (S0, S4–S13) of C. ×hytivus confirms that genomic changes occurred in the very first generations, subsequently slowing down as the process of diploidization is initiated. The duplicated genome of the allopolyploid with double genes from both parents broadens the genetic base of C. ×hytivus, resulting in enhanced phenotypic plasticity. This study provides novel insights into plant polyploid genome evolution and demonstrates a promising strategy for the development of a wide array of novel plant species and varieties through artificial polyploidization.
Original languageEnglish
Article number2004222
JournalAdvanced Science
Volume8
Issue9
ISSN2198-3844
DOIs
Publication statusPublished - May 2021

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