Whole-genome expression analysis of human mesenchymal stromal cells exposed to ultrasmooth tantalum vs. titanium oxide surfaces

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  • Claudia Stiehler, Technische Universität Dresden
  • ,
  • Cody Bünger
  • ,
  • Rupert W. Overall, Technische Universität Dresden
  • ,
  • Loïc Royer, Technische Universität Dresden
  • ,
  • Michael Schroeder
  • Morten Foss
  • Flemming Besenbacher
  • Mogens Kruhøffer, AROS Applied Biotechnology A/S
  • ,
  • Moustapha Kassem, University of Southern Denmark, Stem Cell Unit, King Saud University
  • ,
  • Klaus Peter Günther, Technische Universität Dresden
  • ,
  • Maik Stiehler, Technische Universität Dresden

Durable osseointegration of metallic bone implants requires that progenitor cells attach, proliferate and differentiate on the implant surface. Previously, we demonstrated superior biocompatibility of human mesenchymal stromal cells (MSCs) cultivated on ultrasmooth tantalum (Ta) as compared to titanium (Ti) surface. The aim of this study was to extend the previous investigation of biocompatibility by monitoring temporal gene expression of MSCs on topographically comparable smooth Ta and Ti surfaces using whole-genome gene expression analysis. Total RNA samples from telomerase-immortalized human MSCs cultivated on plain sputter-coated surfaces of Ta or Ti for 1, 2, 4, and 8 days were hybridized to n = 16 U133 Plus 2.0 arrays (Affymetrix®). Functional annotation, cluster and pathway analyses were performed. The vast majority of genes were differentially regulated after 4 days of cultivation and genes upregulated by MSCs exposed to Ta and Ti were predominantly related to the processes of differentiation and transcription, respectively. Functional annotation analysis of the 1,000 temporally most significantly regulated genes suggests earlier cellular differentiation on Ta compared to Ti surface. Key genes related to osteogenesis and cell adhesion were upregulated by MSCs exposed to Ta. We further identified differentially regulated candidate transcription factors, e.g., NRF2, EGR1, IRF-1, IRF-8, NF-Y, and p53 as well as relevant signaling pathways, e.g., p53 and mTOR, indicating e.g., differences in the Ta- and Ti-induced oxidative stress reactions at the cell/biomaterial interface. These findings suggest that Ta is a promising material for bone implants.

Original languageEnglish
JournalCellular and Molecular Bioengineering
Volume6
Issue2
Pages (from-to)199-209
Number of pages11
ISSN1865-5025
DOIs
Publication statusPublished - 1 Jun 2013

    Research areas

  • Biocompatibility, Cell culture, Cell differentiation, Cell proliferation, EGR1, Gene expression, IRF-1, IRF-8, Mesenchymal stem cell, NF-Y, NRF2, Osteoblast, Oxidative stress, p53, Pathway analysis, Tantalum, Titanium

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