Wheat bread making (WBM)-like seed proteins (WSPN): A new family of small prolamins in barley

O. A. Andrzejczak; E. Olesen; S. D.H. Nielsen; L. Tóth; C. K. Madsen; L. Pedersen; N. A. Poulsen; U. Kidmose; L. B. Larsen; K. H. Hebelstrup

doi:10.1016/j.jcs.2024.103961

Wheat bread making (WBM)-like seed proteins (WSPN): A new family of small prolamins in barley

O. A. Andrzejczak, E. Olesen, S. D.H. Nielsen, L. Tóth, C. K. Madsen, L. Pedersen, N. A. Poulsen, U. Kidmose, L. B. Larsen, K. H. Hebelstrup^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

Expression level of the gene Wheat Breadmaking (TaWBM) has been shown to be correlated with bread quality, and influences the rising of the bread loaf. We found that a family of the wbm homologous genes are present not only in wheat, but also in oat, rye and barley. However, a WBM protein has never been demonstrated experimentally. We have identified the existence of a family of WBM-like Seed Proteins (WSPN) from the prolamin fraction of barley flour, encoded by three closely linked genes on the long arm of chromosome 7H, analysing the gene expression by qPCR and relative protein levels by mass spectroscopy. All three genes are expressed specifically in seeds with no detectable expression in the leaves. The barley WSPNs share a common gene structure with a single exon containing an open reading frame in the size 253–313 bp, with the first 80 bp predicted by TargetP 2.0 to encode a sorting peptide. Furthermore, they all contain the conserved motif C–P-X-G-X4-C-X(4–8)-C-X-C. Structure prediction with Alphafold 2.0 suggest that this motif is a structurally conserved microdomain consisting of two antiparallel strands where the cysteines align.

Original language	English
Article number	103961
Journal	Journal of Cereal Science
Volume	118
ISSN	0733-5210
DOIs	https://doi.org/10.1016/j.jcs.2024.103961
Publication status	Published - Jul 2024

Keywords

Baking
Barley
Cysteine network
Prolamins
Wheat wbm

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title = "Wheat bread making (WBM)-like seed proteins (WSPN): A new family of small prolamins in barley",

abstract = "Expression level of the gene Wheat Breadmaking (TaWBM) has been shown to be correlated with bread quality, and influences the rising of the bread loaf. We found that a family of the wbm homologous genes are present not only in wheat, but also in oat, rye and barley. However, a WBM protein has never been demonstrated experimentally. We have identified the existence of a family of WBM-like Seed Proteins (WSPN) from the prolamin fraction of barley flour, encoded by three closely linked genes on the long arm of chromosome 7H, analysing the gene expression by qPCR and relative protein levels by mass spectroscopy. All three genes are expressed specifically in seeds with no detectable expression in the leaves. The barley WSPNs share a common gene structure with a single exon containing an open reading frame in the size 253–313 bp, with the first 80 bp predicted by TargetP 2.0 to encode a sorting peptide. Furthermore, they all contain the conserved motif C–P-X-G-X4-C-X(4–8)-C-X-C. Structure prediction with Alphafold 2.0 suggest that this motif is a structurally conserved microdomain consisting of two antiparallel strands where the cysteines align.",

keywords = "Baking, Barley, Cysteine network, Prolamins, Wheat wbm",

author = "Andrzejczak, {O. A.} and E. Olesen and Nielsen, {S. D.H.} and L. T{\'o}th and Madsen, {C. K.} and L. Pedersen and Poulsen, {N. A.} and U. Kidmose and Larsen, {L. B.} and Hebelstrup, {K. H.}",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors",

year = "2024",

month = jul,

doi = "10.1016/j.jcs.2024.103961",

language = "English",

volume = "118",

journal = "Journal of Cereal Science",

issn = "0733-5210",

publisher = "Academic Press",

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TY - JOUR

T1 - Wheat bread making (WBM)-like seed proteins (WSPN)

T2 - A new family of small prolamins in barley

AU - Andrzejczak, O. A.

AU - Olesen, E.

AU - Nielsen, S. D.H.

AU - Tóth, L.

AU - Madsen, C. K.

AU - Pedersen, L.

AU - Poulsen, N. A.

AU - Kidmose, U.

AU - Larsen, L. B.

AU - Hebelstrup, K. H.

PY - 2024/7

Y1 - 2024/7

N2 - Expression level of the gene Wheat Breadmaking (TaWBM) has been shown to be correlated with bread quality, and influences the rising of the bread loaf. We found that a family of the wbm homologous genes are present not only in wheat, but also in oat, rye and barley. However, a WBM protein has never been demonstrated experimentally. We have identified the existence of a family of WBM-like Seed Proteins (WSPN) from the prolamin fraction of barley flour, encoded by three closely linked genes on the long arm of chromosome 7H, analysing the gene expression by qPCR and relative protein levels by mass spectroscopy. All three genes are expressed specifically in seeds with no detectable expression in the leaves. The barley WSPNs share a common gene structure with a single exon containing an open reading frame in the size 253–313 bp, with the first 80 bp predicted by TargetP 2.0 to encode a sorting peptide. Furthermore, they all contain the conserved motif C–P-X-G-X4-C-X(4–8)-C-X-C. Structure prediction with Alphafold 2.0 suggest that this motif is a structurally conserved microdomain consisting of two antiparallel strands where the cysteines align.

AB - Expression level of the gene Wheat Breadmaking (TaWBM) has been shown to be correlated with bread quality, and influences the rising of the bread loaf. We found that a family of the wbm homologous genes are present not only in wheat, but also in oat, rye and barley. However, a WBM protein has never been demonstrated experimentally. We have identified the existence of a family of WBM-like Seed Proteins (WSPN) from the prolamin fraction of barley flour, encoded by three closely linked genes on the long arm of chromosome 7H, analysing the gene expression by qPCR and relative protein levels by mass spectroscopy. All three genes are expressed specifically in seeds with no detectable expression in the leaves. The barley WSPNs share a common gene structure with a single exon containing an open reading frame in the size 253–313 bp, with the first 80 bp predicted by TargetP 2.0 to encode a sorting peptide. Furthermore, they all contain the conserved motif C–P-X-G-X4-C-X(4–8)-C-X-C. Structure prediction with Alphafold 2.0 suggest that this motif is a structurally conserved microdomain consisting of two antiparallel strands where the cysteines align.

KW - Baking

KW - Barley

KW - Cysteine network

KW - Prolamins

KW - Wheat wbm

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DO - 10.1016/j.jcs.2024.103961

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ER -

Wheat bread making (WBM)-like seed proteins (WSPN): A new family of small prolamins in barley

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