Water Uptake of Airborne Cells of  P. syringae Measured with a Hygroscopicity Tandem Differential Mobility Analyzer

Lærke Sloth Nielsen; Tina Šantl-Temkiv; María Palomeque Sánchez; Andreas Massling; Josephine Caroline Ward; Pia Bomholt Jensen; Thomas Boesen; Markus Petters; Kai Finster; Merete Bilde; Bernadette Rosati

doi:10.1021/acs.est.4c01817

Water Uptake of Airborne Cells of P. syringae Measured with a Hygroscopicity Tandem Differential Mobility Analyzer

Lærke Sloth Nielsen, Tina Šantl-Temkiv, María Palomeque Sánchez, Andreas Massling, Josephine Caroline Ward, Pia Bomholt Jensen, Thomas Boesen, Markus Petters, Kai Finster, Merete Bilde^*, Bernadette Rosati^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Airborne microorganisms impact cloud formation and are involved in disease spreading. The ability of airborne cells to survive and express genes may be limited by reduced water availability in the atmosphere and depend on the ability of the cells to attract water vapor at subsaturated conditions, i.e., their hygroscopicity. We assessed hygroscopic properties of the plant pathogen Pseudomonas syringae, known to participate in cloud formation. We used a hygroscopicity tandem differential mobility analyzer to examine both hydration and dehydration behavior in the relative humidity (RH) range 5-90%. The cells were aerosolized either from Milli-Q water or from a 35 g L -1 NaCl solution, resulting in pure cells or cells associated with NaCl. Pure cells exhibited no deliquescence/efflorescence and a small gradual water uptake reaching a maximum growth factor (GF) of 1.09 ± 0.01 at 90% RH. For cells associated with NaCl, we observed deliquescence and a much larger maximum GF of 1.74 ± 0.03 at 90% RH. Deliquescence RH was comparable to that of pure NaCl, highlighting the major role of the salt associated with the cells. It remains to be investigated how the observed hygroscopic properties relate to survival, metabolic, and ice-nucleation activities of airborne P. syringae.

Original language	English
Journal	Environmental Science & Technology
Volume	58
Issue	43
Pages (from-to)	19211-19221
Number of pages	11
ISSN	0013-936X
DOIs	https://doi.org/10.1021/acs.est.4c01817
Publication status	Published - 29 Oct 2024

Keywords

HTDMA
bioaerosols
clouds
hygroscopic growth
ice nucleation-active bacteria

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10.1021/acs.est.4c01817

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Nielsen, L. S., Šantl-Temkiv, T., Palomeque Sánchez, M., Massling, A., Ward, J. C., Jensen, P. B., Boesen, T., Petters, M., Finster, K., Bilde, M., & Rosati, B. (2024). Water Uptake of Airborne Cells of P. syringae Measured with a Hygroscopicity Tandem Differential Mobility Analyzer. Environmental Science & Technology, 58(43), 19211-19221. https://doi.org/10.1021/acs.est.4c01817

@article{a92a9504924c40e0b178a52eac52b60e,

title = "Water Uptake of Airborne Cells of P. syringae Measured with a Hygroscopicity Tandem Differential Mobility Analyzer",

abstract = "Airborne microorganisms impact cloud formation and are involved in disease spreading. The ability of airborne cells to survive and express genes may be limited by reduced water availability in the atmosphere and depend on the ability of the cells to attract water vapor at subsaturated conditions, i.e., their hygroscopicity. We assessed hygroscopic properties of the plant pathogen Pseudomonas syringae, known to participate in cloud formation. We used a hygroscopicity tandem differential mobility analyzer to examine both hydration and dehydration behavior in the relative humidity (RH) range 5-90%. The cells were aerosolized either from Milli-Q water or from a 35 g L -1 NaCl solution, resulting in pure cells or cells associated with NaCl. Pure cells exhibited no deliquescence/efflorescence and a small gradual water uptake reaching a maximum growth factor (GF) of 1.09 ± 0.01 at 90% RH. For cells associated with NaCl, we observed deliquescence and a much larger maximum GF of 1.74 ± 0.03 at 90% RH. Deliquescence RH was comparable to that of pure NaCl, highlighting the major role of the salt associated with the cells. It remains to be investigated how the observed hygroscopic properties relate to survival, metabolic, and ice-nucleation activities of airborne P. syringae. ",

keywords = "HTDMA, bioaerosols, clouds, hygroscopic growth, ice nucleation-active bacteria",

author = "Nielsen, {L{\ae}rke Sloth} and Tina {\v S}antl-Temkiv and {Palomeque S{\'a}nchez}, Mar{\'i}a and Andreas Massling and Ward, {Josephine Caroline} and Jensen, {Pia Bomholt} and Thomas Boesen and Markus Petters and Kai Finster and Merete Bilde and Bernadette Rosati",

year = "2024",

month = oct,

day = "29",

doi = "10.1021/acs.est.4c01817",

language = "English",

volume = "58",

pages = "19211--19221",

journal = "Environmental Science & Technology",

issn = "0013-936X",

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Nielsen, LS, Šantl-Temkiv, T, Palomeque Sánchez, M, Massling, A , Ward, JC , Jensen, PB , Boesen, T, Petters, M, Finster, K , Bilde, M & Rosati, B 2024, 'Water Uptake of Airborne Cells of P. syringae Measured with a Hygroscopicity Tandem Differential Mobility Analyzer', Environmental Science & Technology, vol. 58, no. 43, pp. 19211-19221. https://doi.org/10.1021/acs.est.4c01817

Water Uptake of Airborne Cells of P. syringae Measured with a Hygroscopicity Tandem Differential Mobility Analyzer. / Nielsen, Lærke Sloth; Šantl-Temkiv, Tina; Palomeque Sánchez, María et al.
In: Environmental Science & Technology, Vol. 58, No. 43, 29.10.2024, p. 19211-19221.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Water Uptake of Airborne Cells of P. syringae Measured with a Hygroscopicity Tandem Differential Mobility Analyzer

AU - Nielsen, Lærke Sloth

AU - Šantl-Temkiv, Tina

AU - Palomeque Sánchez, María

AU - Massling, Andreas

AU - Ward, Josephine Caroline

AU - Jensen, Pia Bomholt

AU - Boesen, Thomas

AU - Petters, Markus

AU - Finster, Kai

AU - Bilde, Merete

AU - Rosati, Bernadette

PY - 2024/10/29

Y1 - 2024/10/29

N2 - Airborne microorganisms impact cloud formation and are involved in disease spreading. The ability of airborne cells to survive and express genes may be limited by reduced water availability in the atmosphere and depend on the ability of the cells to attract water vapor at subsaturated conditions, i.e., their hygroscopicity. We assessed hygroscopic properties of the plant pathogen Pseudomonas syringae, known to participate in cloud formation. We used a hygroscopicity tandem differential mobility analyzer to examine both hydration and dehydration behavior in the relative humidity (RH) range 5-90%. The cells were aerosolized either from Milli-Q water or from a 35 g L -1 NaCl solution, resulting in pure cells or cells associated with NaCl. Pure cells exhibited no deliquescence/efflorescence and a small gradual water uptake reaching a maximum growth factor (GF) of 1.09 ± 0.01 at 90% RH. For cells associated with NaCl, we observed deliquescence and a much larger maximum GF of 1.74 ± 0.03 at 90% RH. Deliquescence RH was comparable to that of pure NaCl, highlighting the major role of the salt associated with the cells. It remains to be investigated how the observed hygroscopic properties relate to survival, metabolic, and ice-nucleation activities of airborne P. syringae.

AB - Airborne microorganisms impact cloud formation and are involved in disease spreading. The ability of airborne cells to survive and express genes may be limited by reduced water availability in the atmosphere and depend on the ability of the cells to attract water vapor at subsaturated conditions, i.e., their hygroscopicity. We assessed hygroscopic properties of the plant pathogen Pseudomonas syringae, known to participate in cloud formation. We used a hygroscopicity tandem differential mobility analyzer to examine both hydration and dehydration behavior in the relative humidity (RH) range 5-90%. The cells were aerosolized either from Milli-Q water or from a 35 g L -1 NaCl solution, resulting in pure cells or cells associated with NaCl. Pure cells exhibited no deliquescence/efflorescence and a small gradual water uptake reaching a maximum growth factor (GF) of 1.09 ± 0.01 at 90% RH. For cells associated with NaCl, we observed deliquescence and a much larger maximum GF of 1.74 ± 0.03 at 90% RH. Deliquescence RH was comparable to that of pure NaCl, highlighting the major role of the salt associated with the cells. It remains to be investigated how the observed hygroscopic properties relate to survival, metabolic, and ice-nucleation activities of airborne P. syringae.

KW - HTDMA

KW - bioaerosols

KW - clouds

KW - hygroscopic growth

KW - ice nucleation-active bacteria

UR - http://www.scopus.com/inward/record.url?scp=85207137672&partnerID=8YFLogxK

U2 - 10.1021/acs.est.4c01817

DO - 10.1021/acs.est.4c01817

M3 - Journal article

C2 - 39425695

SN - 0013-936X

VL - 58

SP - 19211

EP - 19221

JO - Environmental Science & Technology

JF - Environmental Science & Technology

IS - 43

ER -

Water Uptake of Airborne Cells of P. syringae Measured with a Hygroscopicity Tandem Differential Mobility Analyzer

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