Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome

Grum Gebreyesus; Nina Aagaard Poulsen; Mette Krogh Larsen; Lotte Bach Larsen; Esben Skipper Sørensen; Christian Würtz Heegaard; Albert Johannes Buitenhuis

doi:10.3168/jdsc.2020-0048

Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome

Grum Gebreyesus^*, Nina Aagaard Poulsen, Mette Krogh Larsen, Lotte Bach Larsen, Esben Skipper Sørensen, Christian Würtz Heegaard, Albert Johannes Buitenhuis

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

In human nutrition, bovine milk is an essential source of bioavailable vitamin B ₁₂ and B ₁₂-binding proteins, including transcobalamin. In this study, we estimated genetic parameters for milk content of vitamin B ₁₂ and transcobalamin using milk samples from 341 and 663 Danish Holstein cows, respectively. Additionally, we conducted whole-genome association analysis to identify SNP and genes associated with vitamin B ₁₂ and transcobalamin. Our results indicated moderate to high heritability for vitamin B ₁₂ (0.37 ± 0.18) and transcobalamin (0.61 ± 0.13) content in the Danish Holstein. With a significance threshold of –log ₁₀ P-value > 5.87, significant associations were detected between SNP in Bos taurus autosome (BTA)17 and the log-transformed transcobalamin content of milk; no significant association was detected for vitamin B ₁₂. The significant region in BTA17 was imputed to full sequence for further fine mapping, and the SNP with the most significant associations to transcobalamin were assigned to the transcobalamin 2 (TCN2) gene.

Original language	English
Journal	JDS Communications
Volume	2
Issue	3
Pages (from-to)	127-131
Number of pages	5
ISSN	2666-9102
DOIs	https://doi.org/10.3168/jdsc.2020-0048
Publication status	Published - May 2021

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10.3168/jdsc.2020-0048Licence: CC BY

1-s2.0-S266691022100034X-mainFinal published version, 1.29 MBLicence: CC BY

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title = "Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome",

abstract = "In human nutrition, bovine milk is an essential source of bioavailable vitamin B 12 and B 12-binding proteins, including transcobalamin. In this study, we estimated genetic parameters for milk content of vitamin B 12 and transcobalamin using milk samples from 341 and 663 Danish Holstein cows, respectively. Additionally, we conducted whole-genome association analysis to identify SNP and genes associated with vitamin B 12 and transcobalamin. Our results indicated moderate to high heritability for vitamin B 12 (0.37 ± 0.18) and transcobalamin (0.61 ± 0.13) content in the Danish Holstein. With a significance threshold of –log 10 P-value > 5.87, significant associations were detected between SNP in Bos taurus autosome (BTA)17 and the log-transformed transcobalamin content of milk; no significant association was detected for vitamin B 12. The significant region in BTA17 was imputed to full sequence for further fine mapping, and the SNP with the most significant associations to transcobalamin were assigned to the transcobalamin 2 (TCN2) gene.",

author = "Grum Gebreyesus and Poulsen, {Nina Aagaard} and Larsen, {Mette Krogh} and Larsen, {Lotte Bach} and S{\o}rensen, {Esben Skipper} and {W{\"u}rtz Heegaard}, Christian and Buitenhuis, {Albert Johannes}",

year = "2021",

month = may,

doi = "10.3168/jdsc.2020-0048",

language = "English",

volume = "2",

pages = "127--131",

journal = "JDS Communications ",

issn = "2666-9102",

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Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome. / Gebreyesus, Grum ; Poulsen, Nina Aagaard; Larsen, Mette Krogh et al.
In: JDS Communications , Vol. 2, No. 3, 05.2021, p. 127-131.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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T2 - Genetic variation and genome-wide association with loci along the genome

AU - Gebreyesus, Grum

AU - Poulsen, Nina Aagaard

AU - Larsen, Mette Krogh

AU - Larsen, Lotte Bach

AU - Sørensen, Esben Skipper

AU - Würtz Heegaard, Christian

AU - Buitenhuis, Albert Johannes

PY - 2021/5

Y1 - 2021/5

N2 - In human nutrition, bovine milk is an essential source of bioavailable vitamin B 12 and B 12-binding proteins, including transcobalamin. In this study, we estimated genetic parameters for milk content of vitamin B 12 and transcobalamin using milk samples from 341 and 663 Danish Holstein cows, respectively. Additionally, we conducted whole-genome association analysis to identify SNP and genes associated with vitamin B 12 and transcobalamin. Our results indicated moderate to high heritability for vitamin B 12 (0.37 ± 0.18) and transcobalamin (0.61 ± 0.13) content in the Danish Holstein. With a significance threshold of –log 10 P-value > 5.87, significant associations were detected between SNP in Bos taurus autosome (BTA)17 and the log-transformed transcobalamin content of milk; no significant association was detected for vitamin B 12. The significant region in BTA17 was imputed to full sequence for further fine mapping, and the SNP with the most significant associations to transcobalamin were assigned to the transcobalamin 2 (TCN2) gene.

AB - In human nutrition, bovine milk is an essential source of bioavailable vitamin B 12 and B 12-binding proteins, including transcobalamin. In this study, we estimated genetic parameters for milk content of vitamin B 12 and transcobalamin using milk samples from 341 and 663 Danish Holstein cows, respectively. Additionally, we conducted whole-genome association analysis to identify SNP and genes associated with vitamin B 12 and transcobalamin. Our results indicated moderate to high heritability for vitamin B 12 (0.37 ± 0.18) and transcobalamin (0.61 ± 0.13) content in the Danish Holstein. With a significance threshold of –log 10 P-value > 5.87, significant associations were detected between SNP in Bos taurus autosome (BTA)17 and the log-transformed transcobalamin content of milk; no significant association was detected for vitamin B 12. The significant region in BTA17 was imputed to full sequence for further fine mapping, and the SNP with the most significant associations to transcobalamin were assigned to the transcobalamin 2 (TCN2) gene.

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Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome

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