Variations on a theme: Crystal forms of the amino-acid transporter MhsT

Caroline Neumann, Dorota Focht, Sofia Trampari, Joseph A. Lyons, Poul Nissen*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Abstract

The bacterial amino-acid transporter MhsT from the SLC6A family has been crystallized in complex with different substrates in order to understand the determinants of the substrate specificity of the transporter. Surprisingly, crystals of the different MhsT-substrate complexes showed interrelated but different crystal-packing arrangements. Space-group assignment and structure determination of these different crystal forms present challenging combinations of pseudosymmetry, twinning and translational noncrystallographic symmetry.

Original languageEnglish
JournalActa Crystallographica Section F: Structural Biology Communications
Volume78
IssuePart 8
Pages (from-to)297-305
Number of pages9
ISSN2053-230X
DOIs
Publication statusPublished - Aug 2022

Keywords

  • bacterial amino-acid transporters
  • membrane proteins
  • MhsT
  • pseudomerohedral twinning
  • pseudosymmetry
  • translational NCS
  • translational symmetry
  • X-ray crystallography

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