Validation of candidate genes expression profiles during cold acclimation and freezing in perennial ryegrass

Kristina Jonavičienė; Shamila Abeynayake; Gintaras Brazauskas; Torben Asp

doi:10.1007/978-94-017-9044-4_38

Validation of candidate genes expression profiles during cold acclimation and freezing in perennial ryegrass

Kristina Jonavičienė^*, Shamila Abeynayake, Gintaras Brazauskas, Torben Asp

^*Corresponding author for this work

Research output: Contribution to book/anthology/report/proceeding › Book chapter › Research › peer-review

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Abstract

Sixty perennial ryegrass (Lolium perenne L) plants were cold-acclimated and then subjected to freezing at −10 °C. Quantitative real-time polymerase chain reaction was performed to analyse the expression of six candidate genes during cold-acclimation and freezing periods. Four genes responded similarly in two investigated genotypes during both hardening and freezing periods, while the cold-response genes LpIRI and LOS2 distinguished high induction level. These genes represent valuable candidates for further SNP analysis to identify functional markers for cold tolerance in perennial ryegrass.

Original language	English
Title of host publication	Quantitative Traits Breeding for Multifunctional Grasslands and Turf
Number of pages	6
Publisher	Springer
Publication date	1 Jan 2014
Pages	273-278
ISBN (Print)	9789401790437
ISBN (Electronic)	9789401790444
DOIs	https://doi.org/10.1007/978-94-017-9044-4_38
Publication status	Published - 1 Jan 2014

Keywords

Freezing tolerance
Perennial ryegrass
qPCR
Relative gene expression

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10.1007/978-94-017-9044-4_38

Cite this

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abstract = "Sixty perennial ryegrass (Lolium perenne L) plants were cold-acclimated and then subjected to freezing at −10 °C. Quantitative real-time polymerase chain reaction was performed to analyse the expression of six candidate genes during cold-acclimation and freezing periods. Four genes responded similarly in two investigated genotypes during both hardening and freezing periods, while the cold-response genes LpIRI and LOS2 distinguished high induction level. These genes represent valuable candidates for further SNP analysis to identify functional markers for cold tolerance in perennial ryegrass.",

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Validation of candidate genes expression profiles during cold acclimation and freezing in perennial ryegrass. / Jonavičienė, Kristina; Abeynayake, Shamila; Brazauskas, Gintaras et al.
Quantitative Traits Breeding for Multifunctional Grasslands and Turf. Springer, 2014. p. 273-278.

Research output: Contribution to book/anthology/report/proceeding › Book chapter › Research › peer-review

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T1 - Validation of candidate genes expression profiles during cold acclimation and freezing in perennial ryegrass

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AU - Abeynayake, Shamila

AU - Brazauskas, Gintaras

AU - Asp, Torben

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AB - Sixty perennial ryegrass (Lolium perenne L) plants were cold-acclimated and then subjected to freezing at −10 °C. Quantitative real-time polymerase chain reaction was performed to analyse the expression of six candidate genes during cold-acclimation and freezing periods. Four genes responded similarly in two investigated genotypes during both hardening and freezing periods, while the cold-response genes LpIRI and LOS2 distinguished high induction level. These genes represent valuable candidates for further SNP analysis to identify functional markers for cold tolerance in perennial ryegrass.

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Validation of candidate genes expression profiles during cold acclimation and freezing in perennial ryegrass

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Keywords

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