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UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection

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  • Aldino Viegas, Heinrich Heine University Düsseldorf
  • ,
  • Thibault Viennet
  • Tsyr Yan Yu, Harvard University
  • ,
  • Frank Schumann, Bruker BioSpin GmbH, Germany
  • ,
  • Wolfgang Bermel, Bruker BioSpin GmbH, Germany
  • ,
  • Gerhard Wagner, Harvard University
  • ,
  • Manuel Etzkorn, Heinrich Heine University Düsseldorf, Jülich Research Centre

A growing number of nuclear magnetic resonance (NMR) spectroscopic studies are impaired by the limited information content provided by the standard set of experiments conventionally recorded. This is particularly true for studies of challenging biological systems including large, unstructured, membrane-embedded and/or paramagnetic proteins. Here we introduce the concept of unified time-optimized interleaved acquisition NMR (UTOPIA-NMR) for the unified acquisition of standard high-γ (e.g. 1H) and low-γ (e.g. 13C) detected experiments using a single receiver. Our aim is to activate the high level of polarization and information content distributed on low-γ nuclei without disturbing conventional magnetization transfer pathways. We show that using UTOPIA-NMR we are able to recover nearly all of the normally non-used magnetization without disturbing the standard experiments. In other words, additional spectra, that can significantly increase the NMR insights, are obtained for free. While we anticipate a broad range of possible applications we demonstrate for the soluble protein Bcl-xL (ca. 21 kDa) and for OmpX in nanodiscs (ca. 160 kDa) that UTOPIA-NMR is particularly useful for challenging protein systems including perdeuterated (membrane) proteins.

Original languageEnglish
JournalJournal of Biomolecular NMR
Pages (from-to)9-15
Number of pages7
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 Springer Science+Business Media Dordrecht.

    Research areas

  • C-detection, Interleaved acquisition, Low-γ nuclei, UTOPIA-NMR

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