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Utilization of unlocked nucleic acid (UNA) to enhance siRNA performance in vitro and in vivo

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  • BioSyo UNA

    Submitted manuscript, 1.06 MB, PDF document

DOI

  • Hæmatologisk Afdeling, Aalborg Sygehus
  • Department of Molecular Biology
  • Interdisciplinary Nanoscience Center
  • Department of Molecular Biology
Small interfering RNAs (siRNAs) are now established as a favourite tool to reduce gene expression by RNA interference (RNAi) in mammalian cell culture. However, limitations in potency, duration, delivery and specificity of the gene knockdown (KD) are still major obstacles that need further addressing. Recent studies have successfully improved siRNA performance by the introduction of several types of chemical modifications. Here we explore the effect of incorporating unlocked nucleic acid (UNA) into siRNA designs. The acyclic UNA monomers lack the C2'-C3'-bond of the RNA ribose ring and additively decrease nucleic acid duplex thermostability. We show that UNA-modifications of siRNAs are compatible with efficient RNAi and can improve siRNA performance both in vitro and in vivo. In particular, we find that the destabilizing properties of UNA are well suited to enhance the potency of siRNAs which are heavily modified by other chemical modifications such as locked nucleic acid (LNA), C4'hydroxymethyl-DNA (HM), 2'-O-methyl-RNA (OMe), DNA and 2'-Flouro-DNA (F). Interestingly, we find that naked, but UNA-modified siRNAs have dramatically increased biostability in mice and can induce potent KD in a xenograft model of human pancreas cancer. Hereby UNA constitutes an important type of chemical modification for future siRNA designs.
Original languageEnglish
JournalMolecular BioSystems
Volume6
Issue5
Pages (from-to)862-70
Number of pages9
ISSN1742-206X
DOIs
Publication statusPublished - 2010

    Research areas

  • Animals, Cell Line, Tumor, Humans, Liver Neoplasms, Mice, Molecular Structure, RNA Interference, RNA Stability, RNA, Small Interfering, Xenograft Model Antitumor Assays

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