Using known substructures in protein model building and crystallography

TY - JOUR

T1 - Using known substructures in protein model building and crystallography

AU - Jones, T A

AU - Thirup, S

PY - 1986

Y1 - 1986

N2 - Retinol binding protein can be constructed from a small number of large substructures taken from three unrelated proteins. The known structures are treated as a knowledge base from which one extracts information to be used in molecular modelling when lacking true atomic resolution. This includes the interpretation of electron density maps and modelling homologous proteins. Models can be built into maps more accurately and more quickly. This requires the use of a skeleton representation for the electron density which improves the determination of the initial chain tracing. Fragment-matching can be used to bridge gaps for inserted residues when modelling homologous proteins.

AB - Retinol binding protein can be constructed from a small number of large substructures taken from three unrelated proteins. The known structures are treated as a knowledge base from which one extracts information to be used in molecular modelling when lacking true atomic resolution. This includes the interpretation of electron density maps and modelling homologous proteins. Models can be built into maps more accurately and more quickly. This requires the use of a skeleton representation for the electron density which improves the determination of the initial chain tracing. Fragment-matching can be used to bridge gaps for inserted residues when modelling homologous proteins.

KW - Models, Molecular

KW - Protein Conformation

KW - Retinol-Binding Proteins

KW - Structure-Activity Relationship

KW - X-Ray Diffraction

M3 - Journal article

C2 - 3709525

SN - 0261-4189

VL - 5

SP - 819

EP - 822

JO - E M B O Journal

JF - E M B O Journal

IS - 4

ER -