User Guide for the LORE1 Insertion Mutant Resource

Research output: Contribution to book/anthology/report/proceedingBook chapterResearch

Lotus japonicus is a model legume used in the study of plant-microbe interactions, especially in the field of biological nitrogen fixation due to its ability to enter into a symbiotic relationship with a soil bacterium, Mesorhizobium loti. The LORE1 mutant population is a valuable resource for reverse genetics in L. japonicus due to its non-transgenic nature, high tagging efficiency, and low copy count. Here, we outline a workflow for identifying, ordering, and establishing homozygous LORE1 mutant lines for a gene of interest, LjFls2, including protocols for growth and genotyping of a segregating LORE1 population.

Original languageEnglish
Title of host publicationPlant Genomics. Methods in Molecular Biology
Number of pages11
PublisherHumana Press
Publication year2017
ISBN (print)978-1-4939-7001-8
ISBN (Electronic)978-1-4939-7003-2
Publication statusPublished - 2017
SeriesMethods in molecular biology (Clifton, N.J.)

Bibliographical note

Plant Genomics pp 13-23 is part of the Methods in Molecular Biology book series (MIMB, volume 1610)

    Research areas

  • Journal Article

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ID: 115473515