In the future, agricultural production may be constrained by extreme temperatures, limited water supply and nutrient availability as a result of global climate change. Furthermore, an increase in food demand to fit the needs of an overgrowing human population will inevitably lead to a shortage of land and fertile soils for agriculture. Indoor vertical farms (or multilayer systems) have been suggested as solution to overcome land and resource scarcity, by growing crops in multiple layers in highly controlled environments, solely using light-emitting diodes (LEDs) as lighting sources. Apart from ensuring a crop production independent from the external environment, multilayer systems provide a unique opportunity to study light-driven acclimatory responses. The PhD project aims at generating new understanding on the effects of different light qualities, in particular monochromatic light and ultraviolet (UV) radiation, on morphology, physiology and metabolismof plants for potential use in multilayer systems. Cucumber (Cucumis sativus L.) plants were selected as plant material in a set of experiments aimed at: 1) investigating the effects of monochromatic blue, green and red light on morphology, physiology, transcript accumulation and metabolism of cucumber plants and 2) documenting the effects of supplementary UV radiation on acclimatory responses of cucumber plants grown under different monochromatic light backgrounds. The effects of monochromatic light qualities and UV radiation on plant photosynthesis were assessed using gas exchange and chlorophyll fluorescence, while transcript and metabolite accumulation were measured by RT-qPCR and both targeted and untargeted metabolomics, respectively. Our results show that different monochromatic light backgrounds have different impacts on morphology, physiology and metabolism of cucumber plants. Furthermore, our data confirmed that the response of cucumber plants to UV radiation is highly dependent on the color of the light background and these effects are observed at the morphological, physiological, transcriptional and metabolic level. The findings delivers new basic knowledge about plant and UV light interaction, but can on the other hand contribute to the design of costumer-made lighting schemes for future food production, with the objective of promoting targeted biosynthesis of metabolites and improve overall crop quality.