@article{23fe333230204ee889d83365e96344a3,
title = "Ultrasensitive ctDNA detection for preoperative disease stratification in early-stage lung adenocarcinoma",
abstract = "Circulating tumor DNA (ctDNA) detection can predict clinical risk in early-stage tumors. However, clinical applications are constrained by the sensitivity of clinically validated ctDNA detection approaches. NeXT Personal is a whole-genome-based, tumor-informed platform that has been analytically validated for ultrasensitive ctDNA detection at 1–3 ppm of ctDNA with 99.9% specificity. Through an analysis of 171 patients with early-stage lung cancer from the TRACERx study, we detected ctDNA pre-operatively within 81% of patients with lung adenocarcinoma (LUAD), including 53% of those with pathological TNM (pTNM) stage I disease. ctDNA predicted worse clinical outcome, and patients with LUAD with <80 ppm preoperative ctDNA levels (the 95% limit of detection of a ctDNA detection approach previously published in TRACERx) experienced reduced overall survival compared with ctDNA-negative patients with LUAD. Although prospective studies are needed to confirm the clinical utility of the assay, these data show that our approach has the potential to improve disease stratification in early-stage LUADs.",
author = "Black, {James R.M.} and Gabor Bartha and Abbott, {Charles W.} and Boyle, {Sean M.} and Takahiro Karasaki and Bailiang Li and Rui Chen and Jason Harris and Selvaraju Veeriah and Martina Colopi and Bakir, {Maise Al} and Liu, {Wing Kin} and John Lyle and Navarro, {F{\'a}bio C.P.} and Josette Northcott and Pyke, {Rachel Marty} and Hill, {Mark S.} and Kerstin Thol and Ariana Huebner and Chris Bailey and Colliver, {Emma C.} and Carlos Mart{\'i}nez-Ruiz and Kristiana Grigoriadis and Piotr Pawlik and Moore, {David A.} and Daniele Marinelli and Shutkever, {Oliver G.} and Cian Murphy and Monica Sivakumar and Mathew Thomas and Nikos Kostoulas and Rocco Bilancia and Mo Asif and Alan Kirk and {Le Quesne}, John and Craig Dick and Andrew Kidd and Blyth, {Kevin G.} and Peter Russell and Lily Robinson and Shackcloth, {Michael J.} and Sarah Danson and Madeleine Hewish and Chiara Proli and Pratibha Shah and Nadia Fernandes and Nicholson, {Andrew G.} and Mateo Sokac and Judit Kisistok and Birkbak, {Nicolai J.} and {TRACERx Consortium}",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2025.",
year = "2025",
month = jan,
doi = "10.1038/s41591-024-03216-y",
language = "English",
volume = "31",
pages = "70--76",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Research",
number = "1",
}