Two-Photon Irradiation of an Intracellular Singlet Oxygen Photosensitizer: Achieving Localized Sub-Cellular Excitation in Spatially-Resolved Experiments

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Interdisciplinary Nanoscience Center
  • Department of Chemistry
The response of a given cell to spatially-resolved sub-cellular irradiation of a singlet oxygen photosensitizer (protoporphyrin
IX, PpIX) using a focused laser was assessed. In these experiments, incident light was scattered over a volume greater than
that defi ned by the dimensions of the laser beam as a consequence of the inherent inhomogeneity of the cell. Upon irradiation
at a wavelength readily absorbed by PpIX in a one-photon transition, this scattering of light eliminated any advantage
accrued to the use of focused irradiation. However, upon irradiation at a longer wavelength where PpIX can only absorb light
under non-linear two-photon conditions, meaningful intracellular resolution was achieved in the small spatial domain where
the light intensity was high enough for absorption to occur.
Original languageEnglish
JournalFree Radical Research
Pages (from-to)1383-1397
Publication statusPublished - 2010

See relations at Aarhus University Citationformats

ID: 22427865