Two-Photon Irradiation of an Intracellular Singlet Oxygen Photosensitizer: Achieving Localized Sub-Cellular Excitation in Spatially-Resolved Experiments

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  • Interdisciplinary Nanoscience Center
  • Department of Chemistry
The response of a given cell to spatially-resolved sub-cellular irradiation of a singlet oxygen photosensitizer (protoporphyrin
IX, PpIX) using a focused laser was assessed. In these experiments, incident light was scattered over a volume greater than
that defi ned by the dimensions of the laser beam as a consequence of the inherent inhomogeneity of the cell. Upon irradiation
at a wavelength readily absorbed by PpIX in a one-photon transition, this scattering of light eliminated any advantage
accrued to the use of focused irradiation. However, upon irradiation at a longer wavelength where PpIX can only absorb light
under non-linear two-photon conditions, meaningful intracellular resolution was achieved in the small spatial domain where
the light intensity was high enough for absorption to occur.
Original languageEnglish
JournalFree Radical Research
Volume44
Pages (from-to)1383-1397
ISSN1071-5762
Publication statusPublished - 2010

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