The influences of temperature on xanthan biopolymer assemblies on a two-dimensional surface have been thoroughly studied. High resolution atomic force microscope images show that the xanthan nanofibrils can be used to build up well-dispersed 2D scaffold layer after 1 day annealing at 35 degrees C. By increasing annealing temperatures (60 degrees C, 90 degrees C) of xanthan solutions, the well-dispersed layers can be produced rapidly (6h, 0.5h) with micro-sized pore structures. The xanthan scaffold with pore structures potentially allows accommodating micro-sized cells for tissue engineering.