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Two MicroRNAs Linked to Nodule Infection and Nitrogen-Fixing Ability in the Legume Lotus japonicus

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Two MicroRNAs Linked to Nodule Infection and Nitrogen-Fixing Ability in the Legume Lotus japonicus. / De Luis, Ana; Markmann, Katharina; Cognat, Valérie; Holt, Dennis B; Charpentier, Myriam; Parniske, Martin; Stougaard, Jens; Voinnet, Olivier.

In: Plant Physiology, Vol. 160, No. 4, 12.2012, p. 2137-2154.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

De Luis, A, Markmann, K, Cognat, V, Holt, DB, Charpentier, M, Parniske, M, Stougaard, J & Voinnet, O 2012, 'Two MicroRNAs Linked to Nodule Infection and Nitrogen-Fixing Ability in the Legume Lotus japonicus', Plant Physiology, vol. 160, no. 4, pp. 2137-2154. https://doi.org/10.1104/pp.112.204883

APA

De Luis, A., Markmann, K., Cognat, V., Holt, D. B., Charpentier, M., Parniske, M., ... Voinnet, O. (2012). Two MicroRNAs Linked to Nodule Infection and Nitrogen-Fixing Ability in the Legume Lotus japonicus. Plant Physiology, 160(4), 2137-2154. https://doi.org/10.1104/pp.112.204883

CBE

De Luis A, Markmann K, Cognat V, Holt DB, Charpentier M, Parniske M, Stougaard J, Voinnet O. 2012. Two MicroRNAs Linked to Nodule Infection and Nitrogen-Fixing Ability in the Legume Lotus japonicus. Plant Physiology. 160(4):2137-2154. https://doi.org/10.1104/pp.112.204883

MLA

Vancouver

De Luis A, Markmann K, Cognat V, Holt DB, Charpentier M, Parniske M et al. Two MicroRNAs Linked to Nodule Infection and Nitrogen-Fixing Ability in the Legume Lotus japonicus. Plant Physiology. 2012 Dec;160(4):2137-2154. https://doi.org/10.1104/pp.112.204883

Author

De Luis, Ana ; Markmann, Katharina ; Cognat, Valérie ; Holt, Dennis B ; Charpentier, Myriam ; Parniske, Martin ; Stougaard, Jens ; Voinnet, Olivier. / Two MicroRNAs Linked to Nodule Infection and Nitrogen-Fixing Ability in the Legume Lotus japonicus. In: Plant Physiology. 2012 ; Vol. 160, No. 4. pp. 2137-2154.

Bibtex

@article{872c42df002f4a968fe4a47cef31f7bc,
title = "Two MicroRNAs Linked to Nodule Infection and Nitrogen-Fixing Ability in the Legume Lotus japonicus",
abstract = "Legumes overcome nitrogen shortage by developing root nodules in which symbiotic bacteria fix atmospheric nitrogen in exchange for host-derived carbohydrates and mineral nutrients. Nodule development involves the distinct processes of nodule organogenesis, bacterial infection, and the onset of nitrogen fixation. These entail profound, dynamic gene expression changes, notably contributed to by microRNAs (miRNAs). Here, we used deep-sequencing, candidate-based expression studies and a selection of Lotus japonicus mutants uncoupling different symbiosis stages to identify miRNAs involved in symbiotic nitrogen fixation. Induction of a noncanonical miR171 isoform, which targets the key nodulation transcription factor Nodulation Signaling Pathway2, correlates with bacterial infection in nodules. A second candidate, miR397, is systemically induced in the presence of active, nitrogen-fixing nodules but not in that of noninfected or inactive nodule organs. It is involved in nitrogen fixation-related copper homeostasis and targets a member of the laccase copper protein family. These findings thus identify two miRNAs specifically responding to symbiotic infection and nodule function in legumes.",
author = "{De Luis}, Ana and Katharina Markmann and Val{\'e}rie Cognat and Holt, {Dennis B} and Myriam Charpentier and Martin Parniske and Jens Stougaard and Olivier Voinnet",
year = "2012",
month = "12",
doi = "10.1104/pp.112.204883",
language = "English",
volume = "160",
pages = "2137--2154",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "4",

}

RIS

TY - JOUR

T1 - Two MicroRNAs Linked to Nodule Infection and Nitrogen-Fixing Ability in the Legume Lotus japonicus

AU - De Luis, Ana

AU - Markmann, Katharina

AU - Cognat, Valérie

AU - Holt, Dennis B

AU - Charpentier, Myriam

AU - Parniske, Martin

AU - Stougaard, Jens

AU - Voinnet, Olivier

PY - 2012/12

Y1 - 2012/12

N2 - Legumes overcome nitrogen shortage by developing root nodules in which symbiotic bacteria fix atmospheric nitrogen in exchange for host-derived carbohydrates and mineral nutrients. Nodule development involves the distinct processes of nodule organogenesis, bacterial infection, and the onset of nitrogen fixation. These entail profound, dynamic gene expression changes, notably contributed to by microRNAs (miRNAs). Here, we used deep-sequencing, candidate-based expression studies and a selection of Lotus japonicus mutants uncoupling different symbiosis stages to identify miRNAs involved in symbiotic nitrogen fixation. Induction of a noncanonical miR171 isoform, which targets the key nodulation transcription factor Nodulation Signaling Pathway2, correlates with bacterial infection in nodules. A second candidate, miR397, is systemically induced in the presence of active, nitrogen-fixing nodules but not in that of noninfected or inactive nodule organs. It is involved in nitrogen fixation-related copper homeostasis and targets a member of the laccase copper protein family. These findings thus identify two miRNAs specifically responding to symbiotic infection and nodule function in legumes.

AB - Legumes overcome nitrogen shortage by developing root nodules in which symbiotic bacteria fix atmospheric nitrogen in exchange for host-derived carbohydrates and mineral nutrients. Nodule development involves the distinct processes of nodule organogenesis, bacterial infection, and the onset of nitrogen fixation. These entail profound, dynamic gene expression changes, notably contributed to by microRNAs (miRNAs). Here, we used deep-sequencing, candidate-based expression studies and a selection of Lotus japonicus mutants uncoupling different symbiosis stages to identify miRNAs involved in symbiotic nitrogen fixation. Induction of a noncanonical miR171 isoform, which targets the key nodulation transcription factor Nodulation Signaling Pathway2, correlates with bacterial infection in nodules. A second candidate, miR397, is systemically induced in the presence of active, nitrogen-fixing nodules but not in that of noninfected or inactive nodule organs. It is involved in nitrogen fixation-related copper homeostasis and targets a member of the laccase copper protein family. These findings thus identify two miRNAs specifically responding to symbiotic infection and nodule function in legumes.

U2 - 10.1104/pp.112.204883

DO - 10.1104/pp.112.204883

M3 - Journal article

C2 - 23071252

VL - 160

SP - 2137

EP - 2154

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 4

ER -