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Two MicroRNAs Linked to Nodule Infection and Nitrogen-Fixing Ability in the Legume Lotus japonicus

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  • Ana De Luis, Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, France
  • Katharina Markmann, Denmark
  • Valérie Cognat, Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, France
  • Dennis B Holt, Denmark
  • Myriam Charpentier, Biozentrum Martinsried, Ludwig Maximilians University Munich, Germany
  • Martin Parniske, Biozentrum Martinsried, Ludwig Maximilians University Munich, Germany
  • Jens Stougaard
  • Olivier Voinnet, Swiss Federal Institute of Technology Zurich, Department of Biology, Switzerland
Legumes overcome nitrogen shortage by developing root nodules in which symbiotic bacteria fix atmospheric nitrogen in exchange for host-derived carbohydrates and mineral nutrients. Nodule development involves the distinct processes of nodule organogenesis, bacterial infection, and the onset of nitrogen fixation. These entail profound, dynamic gene expression changes, notably contributed to by microRNAs (miRNAs). Here, we used deep-sequencing, candidate-based expression studies and a selection of Lotus japonicus mutants uncoupling different symbiosis stages to identify miRNAs involved in symbiotic nitrogen fixation. Induction of a noncanonical miR171 isoform, which targets the key nodulation transcription factor Nodulation Signaling Pathway2, correlates with bacterial infection in nodules. A second candidate, miR397, is systemically induced in the presence of active, nitrogen-fixing nodules but not in that of noninfected or inactive nodule organs. It is involved in nitrogen fixation-related copper homeostasis and targets a member of the laccase copper protein family. These findings thus identify two miRNAs specifically responding to symbiotic infection and nodule function in legumes.
Original languageEnglish
JournalPlant Physiology
Volume160
Issue4
Pages (from-to)2137-2154
Number of pages18
ISSN0032-0889
DOIs
Publication statusPublished - Dec 2012

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