Two conformationally distinct α-synuclein oligomers share common epitopes and the ability to impair long-term potentiation

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  • Femke van Diggelen, Crossbeta Biosciences BV, Utrecht, The Netherlands.
  • ,
  • Dean Hrle, Klinik für Anaesthesiologie der Technischen Universität München, Klinikum Rechts der Isar, Munich, Germany.
  • ,
  • Mihaela Apetri, Crossbeta Biosciences BV, Utrecht, The Netherlands.
  • ,
  • Gunna Christiansen
  • Gerhard Rammes, Klinik für Anaesthesiologie der Technischen Universität München, Klinikum Rechts der Isar, Munich, Germany.
  • ,
  • Armand Tepper, Crossbeta Biosciences BV, Utrecht, The Netherlands.
  • ,
  • Daniel Erik Otzen

Parkinson's Disease (PD) is a neurodegenerative disease for which there currently is no cure. Aggregation of the pre-synaptic protein α-synuclein (aSN) into oligomers (αSOs) is believed to play a key role in PD pathology, but little is known about αSO formation in vivo and how they induce neurodegeneration. Both the naturally occurring polyunsaturated fatty acid docosahexaenoic acid (DHA) and the lipid peroxidation product 4-hydroxynonenal (HNE), strongly upregulated during ROS conditions, stimulate the formation of αSOs, highlighting a potential role in PD. Yet, insight into αSOs structure and biological effects is still limited as most oligomer preparations studied to date are heterogeneous in composition. Here we have aggregated aSN in the presence of HNE and DHA and purified the αSOs using size exclusion chromatography. Both compounds stimulate formation of spherical αSOs containing anti-parallel β-sheet structure which have the same shape as unmodified αSOs though ca. 2-fold larger. Furthermore, the yield and stabilities of these oligomers are significantly higher than for unmodified aSN. Both modified and unmodified αSOs permeabilize synthetic vesicles, show high co-localisation with glutamatergic synapses and decrease Long Term Potentiation (LTP), in line with the reported synaptotoxic effects of αSOs. We conclude that DHA- and HNE-αSOs are convenient models for pathogenic disease-associated αSOs in PD.

Original languageEnglish
Article number0213663
JournalPLOS ONE
Volume14
Issue3
Number of pages22
ISSN1932-6203
DOIs
Publication statusPublished - Mar 2019

    Research areas

  • AMYLOID OLIGOMERS, ANTIPARALLEL BETA-SHEET, FATTY-ACIDS, LEWY BODY, MECHANISM, NEURONS, OXIDATIVE STRESS, SECONDARY STRUCTURE, SYNAPTIC DYSFUNCTION, TRANSMISSION

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