TSG-6 transfers proteins between glycosaminoglycans via a Ser28-mediated covalent catalytic mechanism

TY - JOUR

T1 - TSG-6 transfers proteins between glycosaminoglycans via a Ser28-mediated covalent catalytic mechanism

AU - Sanggaard, Kristian W

AU - Sonne-Schmidt, Carsten S

AU - Hansen, Toke Peter Krogager

AU - Kristensen, Torsten

AU - Wisniewski, Hans-Georg

AU - Thøgersen, Ida B

AU - Enghild, Jan J

PY - 2008/12/5

Y1 - 2008/12/5

N2 - Studies of the interaction between Bikunin proteins, tumor necrosis factor-stimulated gene-6 protein (TSG-6), and glycosaminoglycans have revealed a unique catalytic activity where TSG-6/heavy chain 2 transfer heavy chain subunits between glycosaminoglycan chains. The activity is mediated by TSG-6/heavy chain 2 and involves a transient SDS stable interaction between TSG-6 and the heavy chain to be transferred. The focus of this study was to characterize the molecular structure of this cross-link to gain further insight into the catalytic mechanism. The result showed that the C-terminal Asp residue of the heavy chains forms an ester bond to Ser(28) beta-carbon of TSG-6 suggesting that this residue plays a role during catalysis.

AB - Studies of the interaction between Bikunin proteins, tumor necrosis factor-stimulated gene-6 protein (TSG-6), and glycosaminoglycans have revealed a unique catalytic activity where TSG-6/heavy chain 2 transfer heavy chain subunits between glycosaminoglycan chains. The activity is mediated by TSG-6/heavy chain 2 and involves a transient SDS stable interaction between TSG-6 and the heavy chain to be transferred. The focus of this study was to characterize the molecular structure of this cross-link to gain further insight into the catalytic mechanism. The result showed that the C-terminal Asp residue of the heavy chains forms an ester bond to Ser(28) beta-carbon of TSG-6 suggesting that this residue plays a role during catalysis.

KW - Carbon

KW - Catalysis

KW - Cell Adhesion Molecules

KW - Cell Line

KW - Cross-Linking Reagents

KW - Glycosaminoglycans

KW - Humans

KW - Mass Spectrometry

KW - Molecular Conformation

KW - Peptides

KW - Protein Binding

KW - Recombinant Proteins

KW - Serine

KW - Sodium Dodecyl Sulfate

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

U2 - 10.1074/jbc.M804240200

DO - 10.1074/jbc.M804240200

M3 - Journal article

C2 - 18820257

SN - 0021-9258

VL - 283

SP - 33919

EP - 33926

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 49

ER -