Treatment of experimental periodontitis with chlorhexidine as adjuvant to scaling and root planing

Nubia Rosa Prietto; Thiago Marchi Martins; Carolina Dos Santos Santinoni; Natália Marcumini Pola; Edilson Ervolino; Amália Machado Bielemann; Fábio Renato Manzolli Leite

doi:10.1016/j.archoralbio.2019.104600

Treatment of experimental periodontitis with chlorhexidine as adjuvant to scaling and root planing

Nubia Rosa Prietto, Thiago Marchi Martins, Carolina Dos Santos Santinoni, Natália Marcumini Pola, Edilson Ervolino, Amália Machado Bielemann, Fábio Renato Manzolli Leite

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

19 Citations (Scopus)

Abstract

OBJECTIVES: To assess whether subgingival irrigation with 0.12 % or 0.2 % chlorhexidine (CHX) immediately after scaling and root planing (SRP) enhances periodontal tissue repair compared to irrigation with saline solution (control).

MATERIALS AND METHODS: Periodontitis was ligature-induced in rat molars for 7 days. Animals were distributed into three groups: 1) SRP group, SRP and irrigation with 0.9 % saline (n = 30); 2) SRP + 0.12 % CHX group, SRP and irrigation with 0.12 % CHX (n = 30); 3) SRP + 0.2 % CHX group, SRP and irrigation with 0.2 % CHX (n = 30). Animals were killed at 7, 15, and 30 days after treatment. Furcation region was histometrically analyzed to determine the bone area. Immunohistochemical reactions were performed for receptor activator of nuclear factor-kB ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP).

RESULTS: Both chlorhexidine groups presented less inflammation and improved tissue repair along the entire experiment when compared with the SRP group. In the histometric analysis at 7, 15 and 30 days, SRP group (4.58 ± 2.51 mm2, 4.21 ± 1.25 mm2, 3.49 ± 1.48 mm2), showed statistically less bone area than groups SRP + 0.12 % CHX (1.86 ± 1.11 mm2; 0.79 ± 0.27 mm2; 0.34 ± 0.14 mm2) and SRP + 0.2 % CHX (1.14 ± 0.51 mm2; 0.98 ± 0.40 mm2; 0.41 ± 0.21 mm2). Both chlorhexidine concentrations modulated the expression of TRAP, RANKL and OPG.

CONCLUSIONS: Subgingival irrigation with chlorhexidine contributed for a quicker shift from a proinflammatory destructive profile to healing of periodontal tissues.

Original language	English
Article number	104600
Journal	Archives of Oral Biology
Volume	110
Number of pages	6
ISSN	0003-9969
DOIs	https://doi.org/10.1016/j.archoralbio.2019.104600
Publication status	Published - 2020

Keywords

Chlorhexidine
Dental scaling
Periodontal diseases
Periodontitis
Rats
Wound healing

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10.1016/j.archoralbio.2019.104600

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@article{f57433d3848e46a1b42ed2b7bd59f599,

title = "Treatment of experimental periodontitis with chlorhexidine as adjuvant to scaling and root planing",

abstract = "OBJECTIVES: To assess whether subgingival irrigation with 0.12 % or 0.2 % chlorhexidine (CHX) immediately after scaling and root planing (SRP) enhances periodontal tissue repair compared to irrigation with saline solution (control).MATERIALS AND METHODS: Periodontitis was ligature-induced in rat molars for 7 days. Animals were distributed into three groups: 1) SRP group, SRP and irrigation with 0.9 % saline (n = 30); 2) SRP + 0.12 % CHX group, SRP and irrigation with 0.12 % CHX (n = 30); 3) SRP + 0.2 % CHX group, SRP and irrigation with 0.2 % CHX (n = 30). Animals were killed at 7, 15, and 30 days after treatment. Furcation region was histometrically analyzed to determine the bone area. Immunohistochemical reactions were performed for receptor activator of nuclear factor-kB ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP).RESULTS: Both chlorhexidine groups presented less inflammation and improved tissue repair along the entire experiment when compared with the SRP group. In the histometric analysis at 7, 15 and 30 days, SRP group (4.58 ± 2.51 mm2, 4.21 ± 1.25 mm2, 3.49 ± 1.48 mm2), showed statistically less bone area than groups SRP + 0.12 % CHX (1.86 ± 1.11 mm2; 0.79 ± 0.27 mm2; 0.34 ± 0.14 mm2) and SRP + 0.2 % CHX (1.14 ± 0.51 mm2; 0.98 ± 0.40 mm2; 0.41 ± 0.21 mm2). Both chlorhexidine concentrations modulated the expression of TRAP, RANKL and OPG.CONCLUSIONS: Subgingival irrigation with chlorhexidine contributed for a quicker shift from a proinflammatory destructive profile to healing of periodontal tissues.",

keywords = "Chlorhexidine, Dental scaling, Periodontal diseases, Periodontitis, Rats, Wound healing",

author = "Prietto, {Nubia Rosa} and Martins, {Thiago Marchi} and Santinoni, {Carolina Dos Santos} and Pola, {Nat{\'a}lia Marcumini} and Edilson Ervolino and Bielemann, {Am{\'a}lia Machado} and Leite, {F{\'a}bio Renato Manzolli}",

year = "2020",

doi = "10.1016/j.archoralbio.2019.104600",

language = "English",

volume = "110",

journal = "Archives of Oral Biology",

issn = "0003-9969",

publisher = "Elsevier Ltd",

}

Treatment of experimental periodontitis with chlorhexidine as adjuvant to scaling and root planing. / Prietto, Nubia Rosa; Martins, Thiago Marchi; Santinoni, Carolina Dos Santos et al.
In: Archives of Oral Biology, Vol. 110, 104600, 2020.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Treatment of experimental periodontitis with chlorhexidine as adjuvant to scaling and root planing

AU - Prietto, Nubia Rosa

AU - Martins, Thiago Marchi

AU - Santinoni, Carolina Dos Santos

AU - Pola, Natália Marcumini

AU - Ervolino, Edilson

AU - Bielemann, Amália Machado

AU - Leite, Fábio Renato Manzolli

PY - 2020

Y1 - 2020

N2 - OBJECTIVES: To assess whether subgingival irrigation with 0.12 % or 0.2 % chlorhexidine (CHX) immediately after scaling and root planing (SRP) enhances periodontal tissue repair compared to irrigation with saline solution (control).MATERIALS AND METHODS: Periodontitis was ligature-induced in rat molars for 7 days. Animals were distributed into three groups: 1) SRP group, SRP and irrigation with 0.9 % saline (n = 30); 2) SRP + 0.12 % CHX group, SRP and irrigation with 0.12 % CHX (n = 30); 3) SRP + 0.2 % CHX group, SRP and irrigation with 0.2 % CHX (n = 30). Animals were killed at 7, 15, and 30 days after treatment. Furcation region was histometrically analyzed to determine the bone area. Immunohistochemical reactions were performed for receptor activator of nuclear factor-kB ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP).RESULTS: Both chlorhexidine groups presented less inflammation and improved tissue repair along the entire experiment when compared with the SRP group. In the histometric analysis at 7, 15 and 30 days, SRP group (4.58 ± 2.51 mm2, 4.21 ± 1.25 mm2, 3.49 ± 1.48 mm2), showed statistically less bone area than groups SRP + 0.12 % CHX (1.86 ± 1.11 mm2; 0.79 ± 0.27 mm2; 0.34 ± 0.14 mm2) and SRP + 0.2 % CHX (1.14 ± 0.51 mm2; 0.98 ± 0.40 mm2; 0.41 ± 0.21 mm2). Both chlorhexidine concentrations modulated the expression of TRAP, RANKL and OPG.CONCLUSIONS: Subgingival irrigation with chlorhexidine contributed for a quicker shift from a proinflammatory destructive profile to healing of periodontal tissues.

AB - OBJECTIVES: To assess whether subgingival irrigation with 0.12 % or 0.2 % chlorhexidine (CHX) immediately after scaling and root planing (SRP) enhances periodontal tissue repair compared to irrigation with saline solution (control).MATERIALS AND METHODS: Periodontitis was ligature-induced in rat molars for 7 days. Animals were distributed into three groups: 1) SRP group, SRP and irrigation with 0.9 % saline (n = 30); 2) SRP + 0.12 % CHX group, SRP and irrigation with 0.12 % CHX (n = 30); 3) SRP + 0.2 % CHX group, SRP and irrigation with 0.2 % CHX (n = 30). Animals were killed at 7, 15, and 30 days after treatment. Furcation region was histometrically analyzed to determine the bone area. Immunohistochemical reactions were performed for receptor activator of nuclear factor-kB ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP).RESULTS: Both chlorhexidine groups presented less inflammation and improved tissue repair along the entire experiment when compared with the SRP group. In the histometric analysis at 7, 15 and 30 days, SRP group (4.58 ± 2.51 mm2, 4.21 ± 1.25 mm2, 3.49 ± 1.48 mm2), showed statistically less bone area than groups SRP + 0.12 % CHX (1.86 ± 1.11 mm2; 0.79 ± 0.27 mm2; 0.34 ± 0.14 mm2) and SRP + 0.2 % CHX (1.14 ± 0.51 mm2; 0.98 ± 0.40 mm2; 0.41 ± 0.21 mm2). Both chlorhexidine concentrations modulated the expression of TRAP, RANKL and OPG.CONCLUSIONS: Subgingival irrigation with chlorhexidine contributed for a quicker shift from a proinflammatory destructive profile to healing of periodontal tissues.

KW - Chlorhexidine

KW - Dental scaling

KW - Periodontal diseases

KW - Periodontitis

KW - Rats

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=85075119881&partnerID=8YFLogxK

U2 - 10.1016/j.archoralbio.2019.104600

DO - 10.1016/j.archoralbio.2019.104600

M3 - Journal article

C2 - 31759184

SN - 0003-9969

VL - 110

JO - Archives of Oral Biology

JF - Archives of Oral Biology

M1 - 104600

ER -

Treatment of experimental periodontitis with chlorhexidine as adjuvant to scaling and root planing

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