Translational control of TWIST1 expression in MCF-10A cell lines recapitulating breast cancer progression

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  • Maarja-Liisa Nairismägi, Denmark
  • Andrii Vislovukh, Department of Translation Mechanisms, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine
  • Q Meng, The Breast Department of the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, China, China
  • Leslie-Ann Largitte, Laboratoire Epigenetique et Cancer, FRE 3239, Institut Andre Lwoff, Villejuif France, France
  • Cornelia Beldiman, Laboratoire Epigenetique et Cancer, FRE 3239, Institut Andre Lwoff, Villejuif France, France
  • Ernst-Martin Füchtbauer
  • Annick Harel-Bellan, Laboratoire Epigenetique et Cancer, FRE 3239, Institut Andre Lwoff, Villejuif France, France
  • Irina Groisman, Laboratoire Epigenetique et Cancer, FRE 3239, Institut Andre Lwoff, Villejuif France, France
TWIST1 is a highly conserved basic helix-loop-helix transcription factor that promotes epithelial–mesenchymal transition (EMT). Its misregulation has been observed in various types of tumors. Using the MCF-10A-series of cell lines that recapitulate the early stages of breast cancer formation and EMT, we found TWIST1 to be upregulated during EMT and downregulated early in carcinogenesis. The TWIST1 3′UTR contains putative regulatory elements, including miRNA target sites and two cytoplasmic polyadenylation elements (CPE). We found that miR-580, CPEB1, and CPEB2 act as negative regulators of TWIST1 expression in a sequence-specific and additive/cooperative manner.
Original languageEnglish
JournalOncogene
Volume31
Pages (from-to)4960-4966
Number of pages6
ISSN0950-9232
DOIs
Publication statusPublished - 2012

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