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Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Standard

Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots. / Hayashi, Satomi; Reid, Dugald; Lorenc, Michał T et al.

In: Plant Biotechnology Journal, Vol. 10, No. 8, 10.2012, p. 995-1010.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Harvard

Hayashi, S, Reid, D, Lorenc, MT, Stiller, J, Edwards, D, Gresshoff, PM & Ferguson, BJ 2012, 'Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots', Plant Biotechnology Journal, vol. 10, no. 8, pp. 995-1010. https://doi.org/10.1111/j.1467-7652.2012.00729.x

APA

Hayashi, S., Reid, D., Lorenc, M. T., Stiller, J., Edwards, D., Gresshoff, P. M., & Ferguson, B. J. (2012). Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots. Plant Biotechnology Journal, 10(8), 995-1010. https://doi.org/10.1111/j.1467-7652.2012.00729.x

CBE

Hayashi S, Reid D, Lorenc MT, Stiller J, Edwards D, Gresshoff PM, Ferguson BJ. 2012. Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots. Plant Biotechnology Journal. 10(8):995-1010. https://doi.org/10.1111/j.1467-7652.2012.00729.x

MLA

Hayashi, Satomi et al. "Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots". Plant Biotechnology Journal. 2012, 10(8). 995-1010. https://doi.org/10.1111/j.1467-7652.2012.00729.x

Vancouver

Hayashi S, Reid D, Lorenc MT, Stiller J, Edwards D, Gresshoff PM et al. Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots. Plant Biotechnology Journal. 2012 Oct;10(8):995-1010. https://doi.org/10.1111/j.1467-7652.2012.00729.x

Author

Hayashi, Satomi ; Reid, Dugald ; Lorenc, Michał T et al. / Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots. In: Plant Biotechnology Journal. 2012 ; Vol. 10, No. 8. pp. 995-1010.

Bibtex

@article{7b0bd2d8edc44ddc8ed51f3722787e2c,
title = "Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots",
abstract = "All lateral organ development in plants, such as nodulation in legumes, requires the temporal and spatial regulation of genes and gene networks. A total mRNA profiling approach using RNA-seq to target the specific soybean (Glycine max) root tissues responding to compatible rhizobia [i.e. the Zone Of Nodulation (ZON)] revealed a large number of novel, often transient, mRNA changes occurring during the early stages of nodulation. Focusing on the ZON enabled us to discard the majority of root tissues and their developmentally diverse gene transcripts, thereby highlighting the lowly and transiently expressed nodulation-specific genes. It also enabled us to concentrate on a precise moment in early nodule development at each sampling time. We focused on discovering genes regulated specifically by the Bradyrhizobium-produced Nod factor signal, by inoculating roots with either a competent wild-type or incompetent mutant (nodC(-) ) strain of Bradyrhizobium japonicum. Collectively, 2915 genes were identified as being differentially expressed, including many known soybean nodulation genes. A number of unknown nodulation gene candidates and soybean orthologues of nodulation genes previously reported in other legume species were also identified. The differential expression of several candidates was confirmed and further characterized via inoculation time-course studies and qRT-PCR. The expression of many genes, including an endo-1,4-β-glucanase, a cytochrome P450 and a TIR-LRR-NBS receptor kinase, was transient, peaking quickly during the initiation of nodule ontogeny. Additional genes were found to be down-regulated. Significantly, a set of differentially regulated genes acting in the gibberellic acid (GA) biosynthesis pathway was discovered, suggesting a novel role of GAs in nodulation.",
keywords = "Bradyrhizobium, Down-Regulation, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Plant, Genes, Plant, Gibberellins, Lipopolysaccharides, Plant Root Nodulation, RNA, Messenger, Root Nodules, Plant, Soybeans, Symbiosis",
author = "Satomi Hayashi and Dugald Reid and Lorenc, {Micha{\l} T} and Jiri Stiller and David Edwards and Gresshoff, {Peter M} and Ferguson, {Brett J}",
note = "{\textcopyright} 2012 The Authors. Plant Biotechnology Journal {\textcopyright} 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.",
year = "2012",
month = oct,
doi = "10.1111/j.1467-7652.2012.00729.x",
language = "English",
volume = "10",
pages = "995--1010",
journal = "Plant Biotechnology Journal",
issn = "1467-7644",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "8",

}

RIS

TY - JOUR

T1 - Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots

AU - Hayashi, Satomi

AU - Reid, Dugald

AU - Lorenc, Michał T

AU - Stiller, Jiri

AU - Edwards, David

AU - Gresshoff, Peter M

AU - Ferguson, Brett J

N1 - © 2012 The Authors. Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.

PY - 2012/10

Y1 - 2012/10

N2 - All lateral organ development in plants, such as nodulation in legumes, requires the temporal and spatial regulation of genes and gene networks. A total mRNA profiling approach using RNA-seq to target the specific soybean (Glycine max) root tissues responding to compatible rhizobia [i.e. the Zone Of Nodulation (ZON)] revealed a large number of novel, often transient, mRNA changes occurring during the early stages of nodulation. Focusing on the ZON enabled us to discard the majority of root tissues and their developmentally diverse gene transcripts, thereby highlighting the lowly and transiently expressed nodulation-specific genes. It also enabled us to concentrate on a precise moment in early nodule development at each sampling time. We focused on discovering genes regulated specifically by the Bradyrhizobium-produced Nod factor signal, by inoculating roots with either a competent wild-type or incompetent mutant (nodC(-) ) strain of Bradyrhizobium japonicum. Collectively, 2915 genes were identified as being differentially expressed, including many known soybean nodulation genes. A number of unknown nodulation gene candidates and soybean orthologues of nodulation genes previously reported in other legume species were also identified. The differential expression of several candidates was confirmed and further characterized via inoculation time-course studies and qRT-PCR. The expression of many genes, including an endo-1,4-β-glucanase, a cytochrome P450 and a TIR-LRR-NBS receptor kinase, was transient, peaking quickly during the initiation of nodule ontogeny. Additional genes were found to be down-regulated. Significantly, a set of differentially regulated genes acting in the gibberellic acid (GA) biosynthesis pathway was discovered, suggesting a novel role of GAs in nodulation.

AB - All lateral organ development in plants, such as nodulation in legumes, requires the temporal and spatial regulation of genes and gene networks. A total mRNA profiling approach using RNA-seq to target the specific soybean (Glycine max) root tissues responding to compatible rhizobia [i.e. the Zone Of Nodulation (ZON)] revealed a large number of novel, often transient, mRNA changes occurring during the early stages of nodulation. Focusing on the ZON enabled us to discard the majority of root tissues and their developmentally diverse gene transcripts, thereby highlighting the lowly and transiently expressed nodulation-specific genes. It also enabled us to concentrate on a precise moment in early nodule development at each sampling time. We focused on discovering genes regulated specifically by the Bradyrhizobium-produced Nod factor signal, by inoculating roots with either a competent wild-type or incompetent mutant (nodC(-) ) strain of Bradyrhizobium japonicum. Collectively, 2915 genes were identified as being differentially expressed, including many known soybean nodulation genes. A number of unknown nodulation gene candidates and soybean orthologues of nodulation genes previously reported in other legume species were also identified. The differential expression of several candidates was confirmed and further characterized via inoculation time-course studies and qRT-PCR. The expression of many genes, including an endo-1,4-β-glucanase, a cytochrome P450 and a TIR-LRR-NBS receptor kinase, was transient, peaking quickly during the initiation of nodule ontogeny. Additional genes were found to be down-regulated. Significantly, a set of differentially regulated genes acting in the gibberellic acid (GA) biosynthesis pathway was discovered, suggesting a novel role of GAs in nodulation.

KW - Bradyrhizobium

KW - Down-Regulation

KW - Gene Expression Regulation, Bacterial

KW - Gene Expression Regulation, Plant

KW - Genes, Plant

KW - Gibberellins

KW - Lipopolysaccharides

KW - Plant Root Nodulation

KW - RNA, Messenger

KW - Root Nodules, Plant

KW - Soybeans

KW - Symbiosis

U2 - 10.1111/j.1467-7652.2012.00729.x

DO - 10.1111/j.1467-7652.2012.00729.x

M3 - Journal article

C2 - 22863334

VL - 10

SP - 995

EP - 1010

JO - Plant Biotechnology Journal

JF - Plant Biotechnology Journal

SN - 1467-7644

IS - 8

ER -