Transformation and regeneration of the legume Lotus corniculatus: A system for molecular studies of symbiotic nitrogen fixation

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  • Annik Petit, Université Paris-Sud 11
  • ,
  • Jens Stougaard
  • Astrid Kühle, Department of Molecular Biology and Plant Physiology
  • ,
  • Kjeld A. Marcker, Department of Molecular Biology and Plant Physiology
  • ,
  • Jacques Tempé, Université Paris-Sud 11

A procedure for transformation and regeneration of the legume species Lotus corniculatus (Bird's-foot trefoil) has been developed. The Agrobacterium rhizogenes 15834 and 8196 strains were used to transform plant cells in wound site infections and transformed roots were propagated in vitro. Transformation was monitored by hybridization with pRi T-DNA sequences and by detection of agropine and mannopine. Transformation frequencies of up to 90% were obtained. Shoots spontaneously formed on hairy root cultures were excised, rooted and inoculated with Rhizobium. Root nodules formed on transformed plants had nitrogenase activities comparable to untransformed nodules. Transcript levels from the nodule-specific leghemoglobin genes and the constitutive ubiquitin genes were similar in transformed and untransformed root nodules. Transformed plants responded to R. loti and Bradyrhizobium sp. (Lotus) strains with phenotypes identical to phenotypes for untransformed plants.

Original languageEnglish
JournalMGG Molecular & General Genetics
Pages (from-to)245-250
Number of pages6
Publication statusPublished - May 1987
Externally publishedYes

    Research areas

  • Agrobacterium rhizogenes, Nitrogen fixation, Plant transformation, Transgenic legumes

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