Transcriptome Analysis of the Response of Burmese Python to Digestion

Jinjie Duan; Kristian Wejse Sanggaard; Leif Schauser; Sanne Enok Lauridsen; Jan J. Enghild; Mikkel Heide Schierup; Tobias Wang

doi:10.1093/gigascience/gix057

Transcriptome Analysis of the Response of Burmese Python to Digestion

Jinjie Duan^*, Kristian Wejse Sanggaard, Leif Schauser, Sanne Enok Lauridsen, Jan J. Enghild, Mikkel Heide Schierup, Tobias Wang

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

19 Citations (Scopus)

330 Downloads (Pure)

Abstract

Exceptional and extreme feeding behaviour makes the Burmese python (Python bivittatus) an interesting model to study physiological remodelling and metabolic adaptation in response to refeeding after prolonged starvation. In this study, we used transcriptome sequencing of 5 visceral organs during fasting as well as 24 hours and 48 hours after ingestion of a large meal to unravel the postprandial changes in Burmese pythons. We first used the pooled data to perform a de novo assembly of the transcriptome and supplemented this with a proteomic survey of enzymes in the plasma and gastric fluid. We constructed a high-quality transcriptome with 34 423 transcripts, of which 19 713 (57%) were annotated. Among highly expressed genes (fragments per kilo base per million sequenced reads > 100 in 1 tissue), we found that the transition from fasting to digestion was associated with differential expression of 43 genes in the heart, 206 genes in the liver, 114 genes in the stomach, 89 genes in the pancreas, and 158 genes in the intestine. We interrogated the function of these genes to test previous hypotheses on the response to feeding. We also used the transcriptome to identify 314 secreted proteins in the gastric fluid of the python. Digestion was associated with an upregulation of genes related to metabolic processes, and translational changes therefore appear to support the postprandial rise in metabolism. We identify stomach-related proteins from a digesting individual and demonstrate that the sensitivity of modern liquid chromatography/tandem mass spectrometry equipment allows the identification of gastric juice proteins that are present during digestion.

Original language	English
Article number	gix057
Journal	GigaScience
Volume	6
Issue	8
Pages (from-to)	1-51
Number of pages	51
ISSN	2047-217X
DOIs	https://doi.org/10.1093/gigascience/gix057
Publication status	Published - 13 Jul 2017

Keywords

Burmese Python
EMBRYONIC CHICKEN PEPSINOGEN
GENE-EXPRESSION
HEART-RATE
METABOLIC-RESPONSE
MOLECULAR-CLONING
MOLURUS
POSTPRANDIAL CARDIAC-HYPERTROPHY
PROTEIN
RNA-SEQ
SNAKES
digestion
pathway
proteome
tissue expression
transcriptome

Access to Document

10.1093/gigascience/gix057

watermarkFinal published version, 1.2 MB

Cite this

@article{fd7abe2ff9fd4742846aa3af10931f50,

title = "Transcriptome Analysis of the Response of Burmese Python to Digestion",

abstract = "Exceptional and extreme feeding behaviour makes the Burmese python (Python bivittatus) an interesting model to study physiological remodelling and metabolic adaptation in response to refeeding after prolonged starvation. In this study, we used transcriptome sequencing of 5 visceral organs during fasting as well as 24 hours and 48 hours after ingestion of a large meal to unravel the postprandial changes in Burmese pythons. We first used the pooled data to perform a de novo assembly of the transcriptome and supplemented this with a proteomic survey of enzymes in the plasma and gastric fluid. We constructed a high-quality transcriptome with 34 423 transcripts, of which 19 713 (57%) were annotated. Among highly expressed genes (fragments per kilo base per million sequenced reads > 100 in 1 tissue), we found that the transition from fasting to digestion was associated with differential expression of 43 genes in the heart, 206 genes in the liver, 114 genes in the stomach, 89 genes in the pancreas, and 158 genes in the intestine. We interrogated the function of these genes to test previous hypotheses on the response to feeding. We also used the transcriptome to identify 314 secreted proteins in the gastric fluid of the python. Digestion was associated with an upregulation of genes related to metabolic processes, and translational changes therefore appear to support the postprandial rise in metabolism. We identify stomach-related proteins from a digesting individual and demonstrate that the sensitivity of modern liquid chromatography/tandem mass spectrometry equipment allows the identification of gastric juice proteins that are present during digestion.",

keywords = "Burmese Python, EMBRYONIC CHICKEN PEPSINOGEN, GENE-EXPRESSION, HEART-RATE, METABOLIC-RESPONSE, MOLECULAR-CLONING, MOLURUS, POSTPRANDIAL CARDIAC-HYPERTROPHY, PROTEIN, RNA-SEQ, SNAKES, digestion, pathway, proteome, tissue expression, transcriptome",

author = "Jinjie Duan and Sanggaard, {Kristian Wejse} and Leif Schauser and Lauridsen, {Sanne Enok} and Enghild, {Jan J.} and Schierup, {Mikkel Heide} and Tobias Wang",

year = "2017",

month = jul,

day = "13",

doi = "10.1093/gigascience/gix057",

language = "English",

volume = "6",

pages = "1--51",

journal = "GigaScience",

issn = "2047-217X",

publisher = "Oxford University Press",

number = "8",

}

TY - JOUR

T1 - Transcriptome Analysis of the Response of Burmese Python to Digestion

AU - Duan, Jinjie

AU - Sanggaard, Kristian Wejse

AU - Schauser, Leif

AU - Lauridsen, Sanne Enok

AU - Enghild, Jan J.

AU - Schierup, Mikkel Heide

AU - Wang, Tobias

PY - 2017/7/13

Y1 - 2017/7/13

N2 - Exceptional and extreme feeding behaviour makes the Burmese python (Python bivittatus) an interesting model to study physiological remodelling and metabolic adaptation in response to refeeding after prolonged starvation. In this study, we used transcriptome sequencing of 5 visceral organs during fasting as well as 24 hours and 48 hours after ingestion of a large meal to unravel the postprandial changes in Burmese pythons. We first used the pooled data to perform a de novo assembly of the transcriptome and supplemented this with a proteomic survey of enzymes in the plasma and gastric fluid. We constructed a high-quality transcriptome with 34 423 transcripts, of which 19 713 (57%) were annotated. Among highly expressed genes (fragments per kilo base per million sequenced reads > 100 in 1 tissue), we found that the transition from fasting to digestion was associated with differential expression of 43 genes in the heart, 206 genes in the liver, 114 genes in the stomach, 89 genes in the pancreas, and 158 genes in the intestine. We interrogated the function of these genes to test previous hypotheses on the response to feeding. We also used the transcriptome to identify 314 secreted proteins in the gastric fluid of the python. Digestion was associated with an upregulation of genes related to metabolic processes, and translational changes therefore appear to support the postprandial rise in metabolism. We identify stomach-related proteins from a digesting individual and demonstrate that the sensitivity of modern liquid chromatography/tandem mass spectrometry equipment allows the identification of gastric juice proteins that are present during digestion.

AB - Exceptional and extreme feeding behaviour makes the Burmese python (Python bivittatus) an interesting model to study physiological remodelling and metabolic adaptation in response to refeeding after prolonged starvation. In this study, we used transcriptome sequencing of 5 visceral organs during fasting as well as 24 hours and 48 hours after ingestion of a large meal to unravel the postprandial changes in Burmese pythons. We first used the pooled data to perform a de novo assembly of the transcriptome and supplemented this with a proteomic survey of enzymes in the plasma and gastric fluid. We constructed a high-quality transcriptome with 34 423 transcripts, of which 19 713 (57%) were annotated. Among highly expressed genes (fragments per kilo base per million sequenced reads > 100 in 1 tissue), we found that the transition from fasting to digestion was associated with differential expression of 43 genes in the heart, 206 genes in the liver, 114 genes in the stomach, 89 genes in the pancreas, and 158 genes in the intestine. We interrogated the function of these genes to test previous hypotheses on the response to feeding. We also used the transcriptome to identify 314 secreted proteins in the gastric fluid of the python. Digestion was associated with an upregulation of genes related to metabolic processes, and translational changes therefore appear to support the postprandial rise in metabolism. We identify stomach-related proteins from a digesting individual and demonstrate that the sensitivity of modern liquid chromatography/tandem mass spectrometry equipment allows the identification of gastric juice proteins that are present during digestion.

KW - Burmese Python

KW - EMBRYONIC CHICKEN PEPSINOGEN

KW - GENE-EXPRESSION

KW - HEART-RATE

KW - METABOLIC-RESPONSE

KW - MOLECULAR-CLONING

KW - MOLURUS

KW - POSTPRANDIAL CARDIAC-HYPERTROPHY

KW - PROTEIN

KW - RNA-SEQ

KW - SNAKES

KW - digestion

KW - pathway

KW - proteome

KW - tissue expression

KW - transcriptome

UR - http://www.scopus.com/inward/record.url?scp=85042150584&partnerID=8YFLogxK

U2 - 10.1093/gigascience/gix057

DO - 10.1093/gigascience/gix057

M3 - Journal article

C2 - 28873961

SN - 2047-217X

VL - 6

SP - 1

EP - 51

JO - GigaScience

JF - GigaScience

IS - 8

M1 - gix057

ER -

Transcriptome Analysis of the Response of Burmese Python to Digestion

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this