Transcript profiling of a bitter variety of narrow-leafed lupin to discover alkaloid biosynthetic genes

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DOI

  • Ting Yang, Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark.
  • ,
  • Istvan Nagy
  • Davide Mancinotti, Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark.
  • ,
  • Sophie Lisa Otterbach, Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark.
  • ,
  • Trine Bundgaard Andersen, Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark.
  • ,
  • Mohammed Saddik Motawia, Section for Plant Biochemistry, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark.
  • ,
  • Torben Asp
  • Fernando Geu-Flores, Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark.

Lupins (Lupinus spp.) are nitrogen-fixing legumes that accumulate toxic alkaloids in their protein-rich beans. These anti-nutritional compounds belong to the family of quinolizidine alkaloids (QAs), which are of interest to the pharmaceutical and chemical industries. To unleash the potential of lupins as protein crops and as sources of QAs, a thorough understanding of the QA pathway is needed. However, only the first enzyme in the pathway, lysine decarboxylase (LDC), is known. Here, we report the transcriptome of a high-QA variety of narrow-leafed lupin (L. angustifolius), obtained using eight different tissues and two different sequencing technologies. In addition, we present a list of 33 genes that are closely co-expressed with LDC and that represent strong candidates for involvement in lupin alkaloid biosynthesis. One of these genes encodes a copper amine oxidase able to convert the product of LDC, cadaverine, into 1-piperideine, as shown by heterologous expression and enzyme assays. Kinetic analysis revealed a low KM value for cadaverine, supporting a role as the second enzyme in the QA pathway. Our transcriptomic data set represents a crucial step towards the discovery of enzymes, transporters, and regulators involved in lupin alkaloid biosynthesis.

Original languageEnglish
JournalJournal of Experimental Botany
Volume68
Issue20
Pages (from-to)5527-5537
Number of pages11
ISSN0022-0957
DOIs
Publication statusPublished - 28 Nov 2017

    Research areas

  • Alkaloid biosynthesis, copper amine oxidase, narrow-leafed lupin, next-generation sequencing, quinolizidine alkaloids, transcript profiling

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