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Toward reliable low-density lipoprotein ultrastructure prediction in clinical conditions: A small-angle X-ray scattering study on individuals with normal and high triglyceride serum levels

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Toward reliable low-density lipoprotein ultrastructure prediction in clinical conditions : A small-angle X-ray scattering study on individuals with normal and high triglyceride serum levels. / Jakubauskas, Dainius; Jansen, Martin; Lyngsø, Jeppe; Cheng, Yuanji; Pedersen, Jan Skov; Cárdenas, Marité.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 31, 102318, 01.2021.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Jakubauskas, Dainius ; Jansen, Martin ; Lyngsø, Jeppe ; Cheng, Yuanji ; Pedersen, Jan Skov ; Cárdenas, Marité. / Toward reliable low-density lipoprotein ultrastructure prediction in clinical conditions : A small-angle X-ray scattering study on individuals with normal and high triglyceride serum levels. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2021 ; Vol. 31.

Bibtex

@article{f239534cd6c343b0af93290c64f93821,
title = "Toward reliable low-density lipoprotein ultrastructure prediction in clinical conditions: A small-angle X-ray scattering study on individuals with normal and high triglyceride serum levels",
abstract = "Atherosclerosis is the main killer in the west and therefore a major health challenge today. Total serum cholesterol and lipoprotein concentrations, used as clinical markers, fail to predict the majority of cases, especially between the risk scale extremes, due to the high complexity in lipoprotein structure and composition. In particular, low-density lipoprotein (LDL) plays a key role in atherosclerosis development, with LDL size being a parameter considered for determining the risk for cardiovascular diseases. Determining LDL size and structural parameters is challenging to address experimentally under physiological-like conditions. This article describes the biochemistry and ultrastructure of normolipidemic and hypertriglyceridemic LDL fractions and subfractions using small-angle X-ray scattering. Our results conclude that LDL particles of hypertriglyceridemic compared to healthy individuals 1) have lower LDL core melting temperature, 2) have lower cholesteryl ester ordering in their core, 3) are smaller, rounder and more spherical below melting temperature, and 4) their protein-containing shell is thinner above melting temperature.",
keywords = "Cardiovascular diseases, Hypertriglyceridemia, LDL structure, LDL subfractionation, Low-density lipoprotein, Small-angle X-ray scattering, SAXS",
author = "Dainius Jakubauskas and Martin Jansen and Jeppe Lyngs{\o} and Yuanji Cheng and Pedersen, {Jan Skov} and Marit{\'e} C{\'a}rdenas",
year = "2021",
month = jan,
doi = "10.1016/j.nano.2020.102318",
language = "English",
volume = "31",
journal = "Nanomedicine: Nanotechnology, Biology and Medicine",
issn = "1549-9634",
publisher = "Elsevier Inc.",

}

RIS

TY - JOUR

T1 - Toward reliable low-density lipoprotein ultrastructure prediction in clinical conditions

T2 - A small-angle X-ray scattering study on individuals with normal and high triglyceride serum levels

AU - Jakubauskas, Dainius

AU - Jansen, Martin

AU - Lyngsø, Jeppe

AU - Cheng, Yuanji

AU - Pedersen, Jan Skov

AU - Cárdenas, Marité

PY - 2021/1

Y1 - 2021/1

N2 - Atherosclerosis is the main killer in the west and therefore a major health challenge today. Total serum cholesterol and lipoprotein concentrations, used as clinical markers, fail to predict the majority of cases, especially between the risk scale extremes, due to the high complexity in lipoprotein structure and composition. In particular, low-density lipoprotein (LDL) plays a key role in atherosclerosis development, with LDL size being a parameter considered for determining the risk for cardiovascular diseases. Determining LDL size and structural parameters is challenging to address experimentally under physiological-like conditions. This article describes the biochemistry and ultrastructure of normolipidemic and hypertriglyceridemic LDL fractions and subfractions using small-angle X-ray scattering. Our results conclude that LDL particles of hypertriglyceridemic compared to healthy individuals 1) have lower LDL core melting temperature, 2) have lower cholesteryl ester ordering in their core, 3) are smaller, rounder and more spherical below melting temperature, and 4) their protein-containing shell is thinner above melting temperature.

AB - Atherosclerosis is the main killer in the west and therefore a major health challenge today. Total serum cholesterol and lipoprotein concentrations, used as clinical markers, fail to predict the majority of cases, especially between the risk scale extremes, due to the high complexity in lipoprotein structure and composition. In particular, low-density lipoprotein (LDL) plays a key role in atherosclerosis development, with LDL size being a parameter considered for determining the risk for cardiovascular diseases. Determining LDL size and structural parameters is challenging to address experimentally under physiological-like conditions. This article describes the biochemistry and ultrastructure of normolipidemic and hypertriglyceridemic LDL fractions and subfractions using small-angle X-ray scattering. Our results conclude that LDL particles of hypertriglyceridemic compared to healthy individuals 1) have lower LDL core melting temperature, 2) have lower cholesteryl ester ordering in their core, 3) are smaller, rounder and more spherical below melting temperature, and 4) their protein-containing shell is thinner above melting temperature.

KW - Cardiovascular diseases

KW - Hypertriglyceridemia

KW - LDL structure

KW - LDL subfractionation

KW - Low-density lipoprotein

KW - Small-angle X-ray scattering

KW - SAXS

UR - http://www.scopus.com/inward/record.url?scp=85094902582&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2020.102318

DO - 10.1016/j.nano.2020.102318

M3 - Journal article

C2 - 33091569

AN - SCOPUS:85094902582

VL - 31

JO - Nanomedicine: Nanotechnology, Biology and Medicine

JF - Nanomedicine: Nanotechnology, Biology and Medicine

SN - 1549-9634

M1 - 102318

ER -