TY - JOUR
T1 - Topography-Guided Proliferation
T2 - Distinct Surface Microtopography Increases Proliferation of Chondrocytes In Vitro
AU - Joergensen, Natasja Leth
AU - Le, Dang Quang Svend
AU - Andersen, Ole Zoffmann
AU - Foss, Morten
AU - Danielsen, Carl Christian
AU - Foldager, Casper Bindzus
AU - Lind, Martin
AU - Lysdahl, Helle
PY - 2015/11
Y1 - 2015/11
N2 - Chondrocyte-based cartilage repair techniques require control of articular chondrocyte expansion ex vivo. Articular chondrocytes have limited availability, and prolonged culturing to obtain a cell number sufficient for clinical use often results in phenotypic alterations and increased costs. In this study, we applied a screening library consisting of micrometer-sized topographical features, termed biosurface structure array (BSSA), to identify specific topographical microstructures affecting the proliferation of human chondrocytes in passage 1 (P1) or 2 (P2). The BSSA library comprised 10 patterns and 16 combinations of pillar size (X) and interpillar gap size (Y). Specific microstructures significantly increased the chondrocytes' proliferative responsiveness in term of patterns, X and Y for P2 compared with P1. The P1 and P2 chondrocytes responded independently to similar patterns after 4 days of culturing, whereas only chondrocytes at P2 responded to specific microstructures with Y=1m and X=2, 4m by a 2.3- and 4.4-fold increased proliferation, respectively. In conclusion, these findings indicate that specific surface topographies promote chondrocyte proliferation and may, indeed, be a tool to control the behavior of chondrocytes in vitro.
AB - Chondrocyte-based cartilage repair techniques require control of articular chondrocyte expansion ex vivo. Articular chondrocytes have limited availability, and prolonged culturing to obtain a cell number sufficient for clinical use often results in phenotypic alterations and increased costs. In this study, we applied a screening library consisting of micrometer-sized topographical features, termed biosurface structure array (BSSA), to identify specific topographical microstructures affecting the proliferation of human chondrocytes in passage 1 (P1) or 2 (P2). The BSSA library comprised 10 patterns and 16 combinations of pillar size (X) and interpillar gap size (Y). Specific microstructures significantly increased the chondrocytes' proliferative responsiveness in term of patterns, X and Y for P2 compared with P1. The P1 and P2 chondrocytes responded independently to similar patterns after 4 days of culturing, whereas only chondrocytes at P2 responded to specific microstructures with Y=1m and X=2, 4m by a 2.3- and 4.4-fold increased proliferation, respectively. In conclusion, these findings indicate that specific surface topographies promote chondrocyte proliferation and may, indeed, be a tool to control the behavior of chondrocytes in vitro.
KW - HUMAN ARTICULAR CHONDROCYTES
KW - CARTILAGE
KW - CELLS
KW - DIFFERENTIATION
KW - TRANSPLANTATION
KW - NANOSCALE
KW - GENES
U2 - 10.1089/ten.tea.2014.0697
DO - 10.1089/ten.tea.2014.0697
M3 - Journal article
SN - 1937-3341
VL - 21
SP - 2757
EP - 2765
JO - Tissue Engineering. Part A
JF - Tissue Engineering. Part A
IS - 21-22
ER -