Tissue-dependent variation of hydrogen sulfide homeostasis in anoxic freshwater turtles

Birgitte Jensen; Sibile Pardue; Christopher G. Kevil; Angela Fago

doi:10.1242/jeb.203976

Tissue-dependent variation of hydrogen sulfide homeostasis in anoxic freshwater turtles

Birgitte Jensen, Sibile Pardue, Christopher G. Kevil, Angela Fago^*

^*Corresponding author for this work

Department of Biology - Zoophysiology

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

Hydrogen sulfide (H₂S) controls numerous physiological responses. To understand its proposed role in metabolic suppression, we measured free H₂S and bound sulfane sulfur (BSS) in tissues of the freshwater turtle Trachemys scripta elegans, a species undergoing strong metabolic suppression when cold and anoxic. In warm normoxic turtles, free H₂S was higher in red blood cells (RBCs) and kidney (∼9–10 µmol l−¹) than in brain, liver and lung (∼1–2 µmol l−¹). These values overall aligned with the tissue H₂S-generating enzymatic activity. BSS levels were similar in all tissues (∼0.5 µmol l−¹) but ∼100-fold higher in RBCs, which have a high thiol content, suggesting that RBCs function as a circulating H₂S reservoir. Cold acclimation caused significant changes in free and bound H₂S in liver, brain and RBCs, but anoxia had no further effect, except in the brain. These results show tissue-dependent sulfide signaling with a potential role in brain metabolic suppression during anoxia in turtles.

Original language	English
Article number	jeb203976
Journal	Journal of Experimental Biology
Volume	222
Issue	12
ISSN	0022-0949
DOIs	https://doi.org/10.1242/jeb.203976
Publication status	Published - Jun 2019

Keywords

Anoxia
Gasotransmitter
Metabolic suppression
Red blood cell
Trachemys scripta

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Tissue-dependent variation of hydrogen sulfide homeostasis in anoxic freshwater turtlesFinal published version, 509 KBLicence: Other

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title = "Tissue-dependent variation of hydrogen sulfide homeostasis in anoxic freshwater turtles",

abstract = "Hydrogen sulfide (H2S) controls numerous physiological responses. To understand its proposed role in metabolic suppression, we measured free H2S and bound sulfane sulfur (BSS) in tissues of the freshwater turtle Trachemys scripta elegans, a species undergoing strong metabolic suppression when cold and anoxic. In warm normoxic turtles, free H2S was higher in red blood cells (RBCs) and kidney (∼9–10 µmol l−1) than in brain, liver and lung (∼1–2 µmol l−1). These values overall aligned with the tissue H2S-generating enzymatic activity. BSS levels were similar in all tissues (∼0.5 µmol l−1) but ∼100-fold higher in RBCs, which have a high thiol content, suggesting that RBCs function as a circulating H2S reservoir. Cold acclimation caused significant changes in free and bound H2S in liver, brain and RBCs, but anoxia had no further effect, except in the brain. These results show tissue-dependent sulfide signaling with a potential role in brain metabolic suppression during anoxia in turtles.",

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doi = "10.1242/jeb.203976",

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Tissue-dependent variation of hydrogen sulfide homeostasis in anoxic freshwater turtles. / Jensen, Birgitte; Pardue, Sibile; Kevil, Christopher G. et al.
In: Journal of Experimental Biology, Vol. 222, No. 12, jeb203976, 06.2019.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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N2 - Hydrogen sulfide (H2S) controls numerous physiological responses. To understand its proposed role in metabolic suppression, we measured free H2S and bound sulfane sulfur (BSS) in tissues of the freshwater turtle Trachemys scripta elegans, a species undergoing strong metabolic suppression when cold and anoxic. In warm normoxic turtles, free H2S was higher in red blood cells (RBCs) and kidney (∼9–10 µmol l−1) than in brain, liver and lung (∼1–2 µmol l−1). These values overall aligned with the tissue H2S-generating enzymatic activity. BSS levels were similar in all tissues (∼0.5 µmol l−1) but ∼100-fold higher in RBCs, which have a high thiol content, suggesting that RBCs function as a circulating H2S reservoir. Cold acclimation caused significant changes in free and bound H2S in liver, brain and RBCs, but anoxia had no further effect, except in the brain. These results show tissue-dependent sulfide signaling with a potential role in brain metabolic suppression during anoxia in turtles.

AB - Hydrogen sulfide (H2S) controls numerous physiological responses. To understand its proposed role in metabolic suppression, we measured free H2S and bound sulfane sulfur (BSS) in tissues of the freshwater turtle Trachemys scripta elegans, a species undergoing strong metabolic suppression when cold and anoxic. In warm normoxic turtles, free H2S was higher in red blood cells (RBCs) and kidney (∼9–10 µmol l−1) than in brain, liver and lung (∼1–2 µmol l−1). These values overall aligned with the tissue H2S-generating enzymatic activity. BSS levels were similar in all tissues (∼0.5 µmol l−1) but ∼100-fold higher in RBCs, which have a high thiol content, suggesting that RBCs function as a circulating H2S reservoir. Cold acclimation caused significant changes in free and bound H2S in liver, brain and RBCs, but anoxia had no further effect, except in the brain. These results show tissue-dependent sulfide signaling with a potential role in brain metabolic suppression during anoxia in turtles.

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Tissue-dependent variation of hydrogen sulfide homeostasis in anoxic freshwater turtles

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