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ThT 101: a primer on the use of thioflavin T to investigate amyloid formation

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  • Kirsten Malmos
  • ,
  • Luis M Blancas-Mejia, Mayo Clinic Rochester, MN
  • ,
  • Benedikt Weber, Technical University of Munich
  • ,
  • Johannes Buchner, Technical University of Munich
  • ,
  • Marina Ramirez-Alvarado, Mayo Clinic Rochester, MN
  • ,
  • Hironobu Naiki, University of Fukui
  • ,
  • Daniel Otzen

Thioflavin T (ThT) has been widely used to investigate amyloid formation since 1989. While concerns have recently been raised about its use as a probe specific for amyloid, ThT still continues to be a very valuable tool for studying kinetic aspects of fibrillation and associated inhibition mechanisms. This review aims to provide a conceptual instruction manual, covering appropriate considerations and pitfalls related to the use of ThT. We start by giving a brief introduction to amyloid formation with focus on the morphology of different aggregate species, followed by a discussion of the quality of protein needed to obtain reliable fibrillation data. After an overview of the photochemical basis for ThT's amyloid binding properties and artifacts that may arise from this, we describe how to plan and analyze ThT assays. We conclude with recommendations for complementary techniques to address shortcomings in the ThT assay.

Original languageEnglish
JournalAmyloid: the Journal of Protein Folding Disorders
Pages (from-to)1-16
Number of pages16
Publication statusPublished - Apr 2017

    Research areas

  • Journal Article

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