Theranostic Tumor Targeted Nanoparticles Combining Drug Delivery with Dual Near Infrared and (19)F Magnetic Resonance Imaging Modalities

TY - JOUR

T1 - Theranostic Tumor Targeted Nanoparticles Combining Drug Delivery with Dual Near Infrared and (19)F Magnetic Resonance Imaging Modalities

AU - Vu-Quang, Hieu

AU - Vinding, Mads Sloth

AU - Nielsen, Thomas

AU - Ullisch, Marcus Görge

AU - Nielsen, Niels Christian

AU - Kjems, Jørgen

N1 - Copyright © 2015. Published by Elsevier Inc.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Combining imaging and drug delivery of "theranostic" nanoparticles has enabled concurrent diagnosis and therapy of diseases. Here, we describe a novel theranostic system that combines two imaging tracers, Perfluorooctyl Bromide (PFOB) for (19)F magnetic resonance imaging (MRI) and Indocyanine Green (ICG) for near infrared (NIR) imaging, with the chemotherapeutic agent Doxorubicin (Dox) into poly (lactic-co-glycolic acid)- poly (ethylene-glycol)-Folate (PLGA-PEG-Folate) nanoparticles. Cell culture studies using flow cytometry, confocal laser scanning microscope imaging, and (19)F MRI showed enhanced uptake of nanoparticles via folate receptors expressed on human nasopharyngeal epidermal carcinoma (KB) cells. In vivo, higher MRI and fluorescence signals were obtained from tumors with (19)F MRI and NIR, respectively, using folate-receptor-targeted nanoparticles compared with non-targeted equivalents. An in vitro cytotoxicity assay showed that folate-targeted nanoparticles were able to kill cancer cells more efficiently than non-folate conjugated particles. Our results suggest a potential use of PLGA-PEG-Folate PFOB/ICG/Dox nanoparticles as a targeted chemotherapy agent traceable by either (19)F MRI or NIR imaging.

AB - Combining imaging and drug delivery of "theranostic" nanoparticles has enabled concurrent diagnosis and therapy of diseases. Here, we describe a novel theranostic system that combines two imaging tracers, Perfluorooctyl Bromide (PFOB) for (19)F magnetic resonance imaging (MRI) and Indocyanine Green (ICG) for near infrared (NIR) imaging, with the chemotherapeutic agent Doxorubicin (Dox) into poly (lactic-co-glycolic acid)- poly (ethylene-glycol)-Folate (PLGA-PEG-Folate) nanoparticles. Cell culture studies using flow cytometry, confocal laser scanning microscope imaging, and (19)F MRI showed enhanced uptake of nanoparticles via folate receptors expressed on human nasopharyngeal epidermal carcinoma (KB) cells. In vivo, higher MRI and fluorescence signals were obtained from tumors with (19)F MRI and NIR, respectively, using folate-receptor-targeted nanoparticles compared with non-targeted equivalents. An in vitro cytotoxicity assay showed that folate-targeted nanoparticles were able to kill cancer cells more efficiently than non-folate conjugated particles. Our results suggest a potential use of PLGA-PEG-Folate PFOB/ICG/Dox nanoparticles as a targeted chemotherapy agent traceable by either (19)F MRI or NIR imaging.

KW - Doxorubicin

KW - F MRI

KW - Folate

KW - ICG

KW - PFOB

KW - PLGA

UR - http://www.scopus.com/inward/record.url?scp=84973667568&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2016.04.010

DO - 10.1016/j.nano.2016.04.010

M3 - Journal article

C2 - 27133191

SN - 1549-9634

VL - 12

SP - 1873

EP - 1884

JO - Nanomedicine: Nanotechnology, Biology and Medicine

JF - Nanomedicine: Nanotechnology, Biology and Medicine

IS - 7

ER -