The XBI BioLab for life science experiments at the European XFEL

Huijong Han; Ekaterina Round; Robin Schubert; Yasmin Gül; Jana Makroczyová; Domingo Meza; Philipp Heuser; Martin Aepfelbacher; Imrich Barák; Christian Betzel; Petra Fromme; Inari Kursula; Poul Nissen; Elena Tereschenko; Joachim Schulz; Charlotte Uetrecht; Jozef Ulicný; Matthias Wilmanns; Janos Hajdu; Victor S Lamzin; Kristina Lorenzen

doi:10.1107/S1600576720013989

The XBI BioLab for life science experiments at the European XFEL

Huijong Han, Ekaterina Round, Robin Schubert, Yasmin Gül, Jana Makroczyová, Domingo Meza, Philipp Heuser, Martin Aepfelbacher, Imrich Barák, Christian Betzel, Petra Fromme, Inari Kursula, Poul Nissen, Elena Tereschenko, Joachim Schulz, Charlotte Uetrecht, Jozef Ulicný, Matthias Wilmanns, Janos Hajdu, Victor S LamzinKristina Lorenzen

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Abstract

The science of X-ray free-electron lasers (XFELs) critically depends on the performance of the X-ray laser and on the quality of the samples placed into the X-ray beam. The stability of biological samples is limited and key biomolecular transformations occur on short timescales. Experiments in biology require a support laboratory in the immediate vicinity of the beamlines. The XBI BioLab of the European XFEL (XBI denotes XFEL Biology Infrastructure) is an integrated user facility connected to the beamlines for supporting a wide range of biological experiments. The laboratory was financed and built by a collaboration between the European XFEL and the XBI User Consortium, whose members come from Finland, Germany, the Slovak Republic, Sweden and the USA, with observers from Denmark and the Russian Federation. Arranged around a central wet laboratory, the XBI BioLab provides facilities for sample preparation and scoring, laboratories for growing prokaryotic and eukaryotic cells, a Bio Safety Level 2 laboratory, sample purification and characterization facilities, a crystallization laboratory, an anaerobic laboratory, an aerosol laboratory, a vacuum laboratory for injector tests, and laboratories for optical microscopy, atomic force microscopy and electron microscopy. Here, an overview of the XBI facility is given and some of the results of the first user experiments are highlighted.

Original language	English
Journal	Journal of Applied Crystallography
Volume	54
Issue	Part 1
Pages (from-to)	7-21
Number of pages	15
ISSN	0021-8898
DOIs	https://doi.org/10.1107/S1600576720013989
Publication status	Published - Feb 2021

Keywords

European XFEL (EuXFEL)
XBI Laboratory
coherent diffractive imaging (CDI)
free-electron lasers (XFELs)
sample preparation and characterization.
serial femtosecond crystallography (SFX)
singleparticle imaging (SPI)
structural biology
time-resolved experiments

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Han, H., Round, E., Schubert, R., Gül, Y., Makroczyová, J., Meza, D., Heuser, P., Aepfelbacher, M., Barák, I., Betzel, C., Fromme, P., Kursula, I., Nissen, P., Tereschenko, E., Schulz, J., Uetrecht, C., Ulicný, J., Wilmanns, M., Hajdu, J., ... Lorenzen, K. (2021). The XBI BioLab for life science experiments at the European XFEL. Journal of Applied Crystallography, 54(Part 1), 7-21. https://doi.org/10.1107/S1600576720013989

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title = "The XBI BioLab for life science experiments at the European XFEL",

abstract = "The science of X-ray free-electron lasers (XFELs) critically depends on the performance of the X-ray laser and on the quality of the samples placed into the X-ray beam. The stability of biological samples is limited and key biomolecular transformations occur on short timescales. Experiments in biology require a support laboratory in the immediate vicinity of the beamlines. The XBI BioLab of the European XFEL (XBI denotes XFEL Biology Infrastructure) is an integrated user facility connected to the beamlines for supporting a wide range of biological experiments. The laboratory was financed and built by a collaboration between the European XFEL and the XBI User Consortium, whose members come from Finland, Germany, the Slovak Republic, Sweden and the USA, with observers from Denmark and the Russian Federation. Arranged around a central wet laboratory, the XBI BioLab provides facilities for sample preparation and scoring, laboratories for growing prokaryotic and eukaryotic cells, a Bio Safety Level 2 laboratory, sample purification and characterization facilities, a crystallization laboratory, an anaerobic laboratory, an aerosol laboratory, a vacuum laboratory for injector tests, and laboratories for optical microscopy, atomic force microscopy and electron microscopy. Here, an overview of the XBI facility is given and some of the results of the first user experiments are highlighted.",

keywords = "European XFEL (EuXFEL), XBI Laboratory, coherent diffractive imaging (CDI), free-electron lasers (XFELs), sample preparation and characterization., serial femtosecond crystallography (SFX), singleparticle imaging (SPI), structural biology, time-resolved experiments",

author = "Huijong Han and Ekaterina Round and Robin Schubert and Yasmin G{\"u}l and Jana Makroczyov{\'a} and Domingo Meza and Philipp Heuser and Martin Aepfelbacher and Imrich Bar{\'a}k and Christian Betzel and Petra Fromme and Inari Kursula and Poul Nissen and Elena Tereschenko and Joachim Schulz and Charlotte Uetrecht and Jozef Ulicn{\'y} and Matthias Wilmanns and Janos Hajdu and Lamzin, {Victor S} and Kristina Lorenzen",

note = "{\textcopyright}",

year = "2021",

month = feb,

doi = "10.1107/S1600576720013989",

language = "English",

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Han, H, Round, E, Schubert, R, Gül, Y, Makroczyová, J, Meza, D, Heuser, P, Aepfelbacher, M, Barák, I, Betzel, C, Fromme, P, Kursula, I, Nissen, P, Tereschenko, E, Schulz, J, Uetrecht, C, Ulicný, J, Wilmanns, M, Hajdu, J, Lamzin, VS & Lorenzen, K 2021, 'The XBI BioLab for life science experiments at the European XFEL', Journal of Applied Crystallography, vol. 54, no. Part 1, pp. 7-21. https://doi.org/10.1107/S1600576720013989

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T1 - The XBI BioLab for life science experiments at the European XFEL

AU - Han, Huijong

AU - Round, Ekaterina

AU - Schubert, Robin

AU - Gül, Yasmin

AU - Makroczyová, Jana

AU - Meza, Domingo

AU - Heuser, Philipp

AU - Aepfelbacher, Martin

AU - Barák, Imrich

AU - Betzel, Christian

AU - Fromme, Petra

AU - Kursula, Inari

AU - Nissen, Poul

AU - Tereschenko, Elena

AU - Schulz, Joachim

AU - Uetrecht, Charlotte

AU - Ulicný, Jozef

AU - Wilmanns, Matthias

AU - Hajdu, Janos

AU - Lamzin, Victor S

AU - Lorenzen, Kristina

N1 - ©

PY - 2021/2

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N2 - The science of X-ray free-electron lasers (XFELs) critically depends on the performance of the X-ray laser and on the quality of the samples placed into the X-ray beam. The stability of biological samples is limited and key biomolecular transformations occur on short timescales. Experiments in biology require a support laboratory in the immediate vicinity of the beamlines. The XBI BioLab of the European XFEL (XBI denotes XFEL Biology Infrastructure) is an integrated user facility connected to the beamlines for supporting a wide range of biological experiments. The laboratory was financed and built by a collaboration between the European XFEL and the XBI User Consortium, whose members come from Finland, Germany, the Slovak Republic, Sweden and the USA, with observers from Denmark and the Russian Federation. Arranged around a central wet laboratory, the XBI BioLab provides facilities for sample preparation and scoring, laboratories for growing prokaryotic and eukaryotic cells, a Bio Safety Level 2 laboratory, sample purification and characterization facilities, a crystallization laboratory, an anaerobic laboratory, an aerosol laboratory, a vacuum laboratory for injector tests, and laboratories for optical microscopy, atomic force microscopy and electron microscopy. Here, an overview of the XBI facility is given and some of the results of the first user experiments are highlighted.

AB - The science of X-ray free-electron lasers (XFELs) critically depends on the performance of the X-ray laser and on the quality of the samples placed into the X-ray beam. The stability of biological samples is limited and key biomolecular transformations occur on short timescales. Experiments in biology require a support laboratory in the immediate vicinity of the beamlines. The XBI BioLab of the European XFEL (XBI denotes XFEL Biology Infrastructure) is an integrated user facility connected to the beamlines for supporting a wide range of biological experiments. The laboratory was financed and built by a collaboration between the European XFEL and the XBI User Consortium, whose members come from Finland, Germany, the Slovak Republic, Sweden and the USA, with observers from Denmark and the Russian Federation. Arranged around a central wet laboratory, the XBI BioLab provides facilities for sample preparation and scoring, laboratories for growing prokaryotic and eukaryotic cells, a Bio Safety Level 2 laboratory, sample purification and characterization facilities, a crystallization laboratory, an anaerobic laboratory, an aerosol laboratory, a vacuum laboratory for injector tests, and laboratories for optical microscopy, atomic force microscopy and electron microscopy. Here, an overview of the XBI facility is given and some of the results of the first user experiments are highlighted.

KW - European XFEL (EuXFEL)

KW - XBI Laboratory

KW - coherent diffractive imaging (CDI)

KW - free-electron lasers (XFELs)

KW - sample preparation and characterization.

KW - serial femtosecond crystallography (SFX)

KW - singleparticle imaging (SPI)

KW - structural biology

KW - time-resolved experiments

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U2 - 10.1107/S1600576720013989

DO - 10.1107/S1600576720013989

M3 - Journal article

C2 - 33833637

SN - 0021-8898

VL - 54

SP - 7

EP - 21

JO - Journal of Applied Crystallography

JF - Journal of Applied Crystallography

IS - Part 1

ER -

The XBI BioLab for life science experiments at the European XFEL

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Keywords

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