The Tr-cp 14 cysteine protease in white clover (Trifolium repens) is localized to the endoplasmic reticulum and is associated with programmed cell death during development of tracheary elements

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The Tr-cp 14 cysteine protease in white clover (Trifolium repens) is localized to the endoplasmic reticulum and is associated with programmed cell death during development of tracheary elements. / Mulisch, Maria; Asp, Torben; Krupinska, Karin; Hollmann, Julien; Holm, Preben Bach.

In: Protoplasma, Vol. 250, No. 2, 04.2013, p. 623-629.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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@article{747e1d52aad147619b86299804b875b1,
title = "The Tr-cp 14 cysteine protease in white clover (Trifolium repens) is localized to the endoplasmic reticulum and is associated with programmed cell death during development of tracheary elements",
abstract = "Cysteine proteases are known to be associated with programmed cell death, developmental senescence and some types of pathogen and stress-induced responses. In the present study, we have characterized the cysteine protease Tr-cp 14 in white clover (Trifolium repens). Tr-cp 14 belongs to the C1A family of cysteine proteases with homology to XCP1 and XCP2 from Arabidopsis thaliana and p48h-17 from Zinnia elegans, which previously have been reported to be associated with tracheary element differentiation. The proform as well as the processed form of the protein was detected in petioles, flowers and leaves, but the processed form was more abundant in leaves and petioles than in flowers. The Tr-cp 14 protein was localized to differentiating tracheary elements within the xylem, indicating that the cysteine protease is involved in protein re-mobilization during tracheary element differentiation. Immunogold studies suggest that the protease prior to the burst of the vacuole was associated to the ER cisternae. After disruption of the tonoplast, it was found in the cytoplasm, and, in later stages, associated with disintegrating material dispersed throughout the cell.",
keywords = "Cysteine protease, Endoplasmic reticulum, Immunolocalization, Tracheary element differentiation, Trifolium repens",
author = "Maria Mulisch and Torben Asp and Karin Krupinska and Julien Hollmann and Holm, {Preben Bach}",
note = "Authors Maria Mulisch and Torben Asp contributed equally to this article. ",
year = "2013",
month = apr,
doi = "10.1007/s00709-012-0427-1",
language = "English",
volume = "250",
pages = "623--629",
journal = "Protoplasma",
issn = "0033-183X",
publisher = "Springer Wien",
number = "2",

}

RIS

TY - JOUR

T1 - The Tr-cp 14 cysteine protease in white clover (Trifolium repens) is localized to the endoplasmic reticulum and is associated with programmed cell death during development of tracheary elements

AU - Mulisch, Maria

AU - Asp, Torben

AU - Krupinska, Karin

AU - Hollmann, Julien

AU - Holm, Preben Bach

N1 - Authors Maria Mulisch and Torben Asp contributed equally to this article.

PY - 2013/4

Y1 - 2013/4

N2 - Cysteine proteases are known to be associated with programmed cell death, developmental senescence and some types of pathogen and stress-induced responses. In the present study, we have characterized the cysteine protease Tr-cp 14 in white clover (Trifolium repens). Tr-cp 14 belongs to the C1A family of cysteine proteases with homology to XCP1 and XCP2 from Arabidopsis thaliana and p48h-17 from Zinnia elegans, which previously have been reported to be associated with tracheary element differentiation. The proform as well as the processed form of the protein was detected in petioles, flowers and leaves, but the processed form was more abundant in leaves and petioles than in flowers. The Tr-cp 14 protein was localized to differentiating tracheary elements within the xylem, indicating that the cysteine protease is involved in protein re-mobilization during tracheary element differentiation. Immunogold studies suggest that the protease prior to the burst of the vacuole was associated to the ER cisternae. After disruption of the tonoplast, it was found in the cytoplasm, and, in later stages, associated with disintegrating material dispersed throughout the cell.

AB - Cysteine proteases are known to be associated with programmed cell death, developmental senescence and some types of pathogen and stress-induced responses. In the present study, we have characterized the cysteine protease Tr-cp 14 in white clover (Trifolium repens). Tr-cp 14 belongs to the C1A family of cysteine proteases with homology to XCP1 and XCP2 from Arabidopsis thaliana and p48h-17 from Zinnia elegans, which previously have been reported to be associated with tracheary element differentiation. The proform as well as the processed form of the protein was detected in petioles, flowers and leaves, but the processed form was more abundant in leaves and petioles than in flowers. The Tr-cp 14 protein was localized to differentiating tracheary elements within the xylem, indicating that the cysteine protease is involved in protein re-mobilization during tracheary element differentiation. Immunogold studies suggest that the protease prior to the burst of the vacuole was associated to the ER cisternae. After disruption of the tonoplast, it was found in the cytoplasm, and, in later stages, associated with disintegrating material dispersed throughout the cell.

KW - Cysteine protease

KW - Endoplasmic reticulum

KW - Immunolocalization

KW - Tracheary element differentiation

KW - Trifolium repens

U2 - 10.1007/s00709-012-0427-1

DO - 10.1007/s00709-012-0427-1

M3 - Journal article

C2 - 22707085

VL - 250

SP - 623

EP - 629

JO - Protoplasma

JF - Protoplasma

SN - 0033-183X

IS - 2

ER -