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The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2

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Tumor necrosis factor-stimulated gene-6 protein (TSG-6) is involved in the transfer of heavy chains (HCs) from inter-alpha-inhibitor (IalphaI), pre-alpha-inhibitor, and as shown here HC2.bikunin to hyaluronan through the formation of covalent HC.TSG-6 intermediates. In contrast to IalphaI and HC2.bikunin, pre-alpha-inhibitor does not form a covalent complex in vitro using purified proteins but needs the presence of another factor (Rugg, M. S., Willis, A. C., Mukhopadhyay, D., Hascall, V. C., Fries, E., Fülöp, C., Milner, C. M., and Day, A. J. (2005) J. Biol. Chem. 280, 25674-25686). In the present study we purified the required component from human plasma and identified it as HC2. Proteins containing HC2 including IalphaI, HC2.bikunin, and free HC2 promoted the formation of HC3.TSG-6 and subsequently HC3.hyaluronan complexes. HC1 or HC3 did not possess this activity. The presented data reveal that both HC2 and TSG-6 are required for the transesterification reactions to occur.
Original languageEnglish
JournalJournal of Biological Chemistry
Volume283
Issue27
Pages (from-to)18530-18537
Number of pages8
ISSN0021-9258
DOIs
Publication statusPublished - 4 Jul 2008

    Research areas

  • Alpha-Globulins, Cell Adhesion Molecules, Humans, Hyaluronic Acid, Male, Multiprotein Complexes

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