The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans

TY - JOUR

T1 - The Tabula Sapiens

T2 - A multiple-organ, single-cell transcriptomic atlas of humans

AU - de Morree, Antoine

AU - Giudice, Linda C

AU - Rando, Thomas A

AU - Tabula Sapiens Consortium

PY - 2022/5/13

Y1 - 2022/5/13

N2 - Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing cell biology and enabling new insights into the physiology of human organs. We created a human reference atlas comprising nearly 500,000 cells from 24 different tissues and organs, many from the same donor. This atlas enabled molecular characterization of more than 400 cell types, their distribution across tissues, and tissue-specific variation in gene expression. Using multiple tissues from a single donor enabled identification of the clonal distribution of T cells between tissues, identification of the tissue-specific mutation rate in B cells, and analysis of the cell cycle state and proliferative potential of shared cell types across tissues. Cell type-specific RNA splicing was discovered and analyzed across tissues within an individual.

AB - Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing cell biology and enabling new insights into the physiology of human organs. We created a human reference atlas comprising nearly 500,000 cells from 24 different tissues and organs, many from the same donor. This atlas enabled molecular characterization of more than 400 cell types, their distribution across tissues, and tissue-specific variation in gene expression. Using multiple tissues from a single donor enabled identification of the clonal distribution of T cells between tissues, identification of the tissue-specific mutation rate in B cells, and analysis of the cell cycle state and proliferative potential of shared cell types across tissues. Cell type-specific RNA splicing was discovered and analyzed across tissues within an individual.

KW - Atlases as Topic

KW - B-Lymphocytes/metabolism

KW - Cells/metabolism

KW - Humans

KW - Organ Specificity/genetics

KW - RNA Splicing

KW - Single-Cell Analysis

KW - T-Lymphocytes/metabolism

KW - Transcriptome

U2 - 10.1126/science.abl4896

DO - 10.1126/science.abl4896

M3 - Journal article

C2 - 35549404

SN - 0036-8075

VL - 376

SP - eabl4896

JO - Science (New York, N.Y.)

JF - Science (New York, N.Y.)

IS - 6594

M1 - abl4896

ER -