Aarhus University Seal

The Role of Nanoscale Distribution of Fibronectin in the Adhesion of Staphylococcus aureus Studied by Protein Patterning and DNA-PAINT

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review


  • Heba Khateb
  • ,
  • Rasmus S. Sørensen
  • ,
  • Kimberly Cramer, Max Planck Institute of Biochemistry
  • ,
  • Alexandra S. Eklund, Max Planck Institute of Biochemistry
  • ,
  • Jorgen Kjems
  • Rikke L. Meyer
  • Ralf Jungmann, Max Planck Institute of Biochemistry, Ludwig Maximilian University of Munich
  • ,
  • Duncan S. Sutherland

Staphylococcus aureus is a widespread and highly virulent pathogen that can cause superficial and invasive infections. Interactions between S. aureus surface receptors and the extracellular matrix protein fibronectin mediate the bacterial invasion of host cells and is implicated in the colonization of medical implant surfaces. In this study, we investigate the role of distribution of both fibronectin and cellular receptors on the adhesion of S. aureus to interfaces as a model for primary adhesion at tissue interfaces or biomaterials. We present fibronectin in patches of systematically varied size (100-1000 nm) in a background of protein and bacteria rejecting chemistry based on PLL-g-PEG and studied S. aureus adhesion under flow. We developed a single molecule imaging assay for localizing fibronectin binding receptors on the surface of S. aureus via the super-resolution DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) technique. Our results indicate that S. aureus adhesion to fibronectin biointerfaces is regulated by the size of available ligand patterns, with an adhesion threshold of 300 nm and larger. DNA-PAINT was used to visualize fibronectin binding receptor organization in situ at ∼7 nm localization precision and with a surface density of 38-46 μm-2, revealing that the engagement of two or more receptors is required for strong S. aureus adhesion to fibronectin biointerfaces.

Original languageEnglish
JournalACS Nano
Pages (from-to)10392-10403
Number of pages12
Publication statusPublished - Jul 2022

Bibliographical note

Publisher Copyright:
© 2022 The Authors. Published by American Chemical Society.

    Research areas

  • colloidal lithography, DNA-PAINT, fibronectin, fibronectin binding protein localization, protein nanopattern, Staphylococcus aureus adhesion

See relations at Aarhus University Citationformats

ID: 286764979