The Role of Complement Activating Collectins and Associated Serine Proteases in Patients With Hematological Malignancies, Receiving High-Dose Chemotherapy, and Autologous Hematopoietic Stem Cell Transplantations (Auto-HSCT)

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

DOI

  • Anna S Świerzko, Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Łódź, Poland.
  • ,
  • Mateusz Michalski, Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Łódź, Poland.
  • ,
  • Anna Sokołowska, Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Łódź, Poland.
  • ,
  • Mateusz Nowicki, Department of Hematology, Copernicus Memorial Hospital in Łódź Comprehensive Cancer Center and Traumatology, Łódź, Poland.
  • ,
  • Łukasz Eppa, Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Łódź, Poland.
  • ,
  • Agnieszka Szala-Poździej, Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Łódź, Poland.
  • ,
  • Iwona Mitrus, Department of Bone Marrow Transplantation and Oncohematology, Cancer Center and Institute of Oncology, Gliwice, Poland.
  • ,
  • Anna Szmigielska-Kapłon, Department of Hematology, Medical University of Łódz, Łódź, Poland.
  • ,
  • Małgorzata Sobczyk-Kruszelnicka, Department of Bone Marrow Transplantation and Oncohematology, Cancer Center and Institute of Oncology, Gliwice, Poland.
  • ,
  • Katarzyna Michalak, Department of Bone Marrow Transplantation and Oncohematology, Cancer Center and Institute of Oncology, Gliwice, Poland.
  • ,
  • Aleksandra Gołos, Department of Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland.
  • ,
  • Agnieszka Wierzbowska, Department of Hematology, Medical University of Łódz, Łódź, Poland.
  • ,
  • Sebastian Giebel, Department of Bone Marrow Transplantation and Oncohematology, Cancer Center and Institute of Oncology, Gliwice, Poland.
  • ,
  • Krzysztof Jamroziak, Department of Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland.
  • ,
  • Marek L Kowalski, Department of Immunology and Allergy, Medical University of Łódz, Łódź, Poland.
  • ,
  • Olga Brzezińska, Department of Rheumatology, Medical University of Łódz, Łódź, Poland.
  • ,
  • Steffen Thiel
  • Jens C Jensenius
  • Katarzyna Kasperkiewicz, Department of Microbiology, University of Silesia, Katowice, Poland.
  • ,
  • Maciej Cedzyński, Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Łódź, Poland.

We conducted a prospective study of 312 patients (194 with multiple myeloma, 118 with lymphomas) receiving high-dose conditioning chemotherapy and autologous hematopoietic stem cell transplantation (auto-HSCT). Polymorphisms of MBL2 and MASP2 genes were investigated and serial measurements of serum concentrations of mannose-binding lectin (MBL), CL-LK collectin and MASP-2 as well as activities of MBL-MASP-1 and MBL-MASP-2 complex were made. Serum samples were taken before conditioning chemotherapy, before HSCT and once weekly after (totally 4-5 samples); in minority of subjects also 1 and/or 3 months post transplantation. The results were compared with data from 267 healthy controls and analyzed in relation to clinical data to explore possible associations with cancer and with chemotherapy-induced medical complications. We found a higher frequency of MBL deficiency-associated genotypes (LXA/O or O/O) among multiple myeloma patients compared with controls. It was however not associated with hospital infections or post-HSCT recovery of leukocytes, but seemed to be associated with the most severe infections during follow-up. Paradoxically, high MBL serum levels were a risk factor for prolonged fever and some infections. The first possible association of MBL2 gene 3'-untranslated region polymorphism with cancer (lymphoma) in Caucasians was noted. Heterozygosity for MASP2 gene +359 A>G mutation was relatively frequent in lymphoma patients who experienced bacteremia during hospital stay. The median concentration of CL-LK was higher in myeloma patients compared with healthy subjects. Chemotherapy induced marked increases in serum MBL and MASP-2 concentrations, prolonged for several weeks and relatively slighter decline in CL-LK level within 1 week. Conflicting findings on the influence of MBL on infections following chemotherapy of myeloma and lymphoma have been reported. Here we found no evidence for an association between MBL deficiency and infection during the short period of neutropenia following conditioning treatment before HSCT. However, we noted a possible protective effect of MBL during follow-up, and suspected that to be fully effective when able to act in combination with phagocytic cells after their recovery.

Original languageEnglish
JournalFrontiers in Immunology
Volume9
Pages (from-to)2153
ISSN1664-3224
DOIs
Publication statusPublished - 2018

See relations at Aarhus University Citationformats

ID: 137653883