The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism. / Juhl, Dennis Wilkens; Glattard, Elise; Lointier, Morane; Bampilis, Panos; Bechinger, Burkhard.

In: Frontiers in cellular and infection microbiology, Vol. 10, 526459, 30.09.2020.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Juhl, DW, Glattard, E, Lointier, M, Bampilis, P & Bechinger, B 2020, 'The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism', Frontiers in cellular and infection microbiology, vol. 10, 526459. https://doi.org/10.3389/fcimb.2020.526459

APA

Juhl, D. W., Glattard, E., Lointier, M., Bampilis, P., & Bechinger, B. (2020). The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism. Frontiers in cellular and infection microbiology, 10, [526459]. https://doi.org/10.3389/fcimb.2020.526459

CBE

Juhl DW, Glattard E, Lointier M, Bampilis P, Bechinger B. 2020. The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism. Frontiers in cellular and infection microbiology. 10:Article 526459. https://doi.org/10.3389/fcimb.2020.526459

MLA

Vancouver

Juhl DW, Glattard E, Lointier M, Bampilis P, Bechinger B. The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism. Frontiers in cellular and infection microbiology. 2020 Sep 30;10. 526459. https://doi.org/10.3389/fcimb.2020.526459

Author

Juhl, Dennis Wilkens ; Glattard, Elise ; Lointier, Morane ; Bampilis, Panos ; Bechinger, Burkhard. / The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism. In: Frontiers in cellular and infection microbiology. 2020 ; Vol. 10.

Bibtex

@article{d4977871cf4946a0946e461b25db2dff,
title = "The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism",
abstract = "Magainin 2 and PGLa are antimicrobial peptides found together in frog skin secretions. When added as a mixture they show an order of magnitude increase in antibacterial activity and in model membrane permeation assays. Here we demonstrate that both peptides can form fibers with beta-sheet/turn signature in ATR-FTIR- and CD-spectroscopic analyses, but with different morphologies in EM images. Whereas, fiber formation results in acute reduction of the antimicrobial activity of the individual peptides, the synergistic enhancement of activity remains for the equimolar mixture of PGLa and magainin 2 also after fibril formation. The biological significance and potential applications of such supramolecular aggregates are discussed.",
keywords = "amyloid fiber, antimicrobial peptide, gradual release, peptide-lipid interaction, supramolecular assembly",
author = "Juhl, {Dennis Wilkens} and Elise Glattard and Morane Lointier and Panos Bampilis and Burkhard Bechinger",
year = "2020",
month = sep,
day = "30",
doi = "10.3389/fcimb.2020.526459",
language = "English",
volume = "10",
journal = "Frontiers in cellular and infection microbiology",
issn = "2235-2988",
publisher = "FRONTIERS MEDIA SA",

}

RIS

TY - JOUR

T1 - The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism

AU - Juhl, Dennis Wilkens

AU - Glattard, Elise

AU - Lointier, Morane

AU - Bampilis, Panos

AU - Bechinger, Burkhard

PY - 2020/9/30

Y1 - 2020/9/30

N2 - Magainin 2 and PGLa are antimicrobial peptides found together in frog skin secretions. When added as a mixture they show an order of magnitude increase in antibacterial activity and in model membrane permeation assays. Here we demonstrate that both peptides can form fibers with beta-sheet/turn signature in ATR-FTIR- and CD-spectroscopic analyses, but with different morphologies in EM images. Whereas, fiber formation results in acute reduction of the antimicrobial activity of the individual peptides, the synergistic enhancement of activity remains for the equimolar mixture of PGLa and magainin 2 also after fibril formation. The biological significance and potential applications of such supramolecular aggregates are discussed.

AB - Magainin 2 and PGLa are antimicrobial peptides found together in frog skin secretions. When added as a mixture they show an order of magnitude increase in antibacterial activity and in model membrane permeation assays. Here we demonstrate that both peptides can form fibers with beta-sheet/turn signature in ATR-FTIR- and CD-spectroscopic analyses, but with different morphologies in EM images. Whereas, fiber formation results in acute reduction of the antimicrobial activity of the individual peptides, the synergistic enhancement of activity remains for the equimolar mixture of PGLa and magainin 2 also after fibril formation. The biological significance and potential applications of such supramolecular aggregates are discussed.

KW - amyloid fiber

KW - antimicrobial peptide

KW - gradual release

KW - peptide-lipid interaction

KW - supramolecular assembly

UR - http://www.scopus.com/inward/record.url?scp=85092723425&partnerID=8YFLogxK

U2 - 10.3389/fcimb.2020.526459

DO - 10.3389/fcimb.2020.526459

M3 - Journal article

C2 - 33102247

AN - SCOPUS:85092723425

VL - 10

JO - Frontiers in cellular and infection microbiology

JF - Frontiers in cellular and infection microbiology

SN - 2235-2988

M1 - 526459

ER -