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The proteome of seed development in the model legume Lotus japonicus

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  • Svend Dam, Denmark
  • Brian S Laursen, Denmark
  • Jane H Ornfelt, Denmark
  • Bjarne Jochimsen, Denmark
  • Hans Henrik Stærfeldt, Denmark
  • Carsten Friis, Denmark
  • Kasper Nielsen, Denmark
  • Nicolas Goffard, Denmark
  • Soren Besenbacher, Denmark
  • Lene Krusell, Denmark
  • Shusei Sato, Denmark
  • Satoshi Tabata, Denmark
  • Ida Thøgersen
  • Jan J Enghild
  • Jens Stougaard
We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean and pea, Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic three developmental phases of legume seeds and the presence of embryo, endosperm and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined and this indicates that the composition of mature Lotus seed is more similar to soybean than pea. In a first attempt to determine the seed proteome both a 2-D PAGE approach and a GeLC-MS/MS approach were used. Globulins were analyzed by 2-D PAGE and five legumins, LLP1-5, and two convicilins, LCP1-2, were identified by MALDI Q-TOF mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a GeLC-MS/MS approach was used, and 665 and 181 unique proteins corresponding to gene accession numbers were identified for the two phases respectively. All the proteome data including the experimental data and MS spectra peaks were collected in a database that is available to the scientific community via a web-interface (http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi). This database establishes the basis for relating physiology, biochemistry and regulation of seed development in Lotus. Together with a new a web-interface (http://bioinfoserver.rsbs.anu.edu.au/utils/PathExpress4legumes/) collecting all protein identifications for Lotus, Medicago, and soybean seed proteomes, this database is a valuable resource for comparative seed proteomics and pathway analysis within and beyond the legume family.
Original languageEnglish
JournalPlant Physiology
Publication statusPublished - 2009

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