TY - JOUR
T1 - The proteome of seed development in the model legume Lotus japonicus
AU - Dam, Svend
AU - Laursen, Brian S
AU - Ornfelt, Jane H
AU - Jochimsen, Bjarne
AU - Stærfeldt, Hans Henrik
AU - Friis, Carsten
AU - Nielsen, Kasper
AU - Goffard, Nicolas
AU - Besenbacher, Soren
AU - Krusell, Lene
AU - Sato, Shusei
AU - Tabata, Satoshi
AU - Thøgersen, Ida
AU - Enghild, Jan J
AU - Stougaard, Jens
PY - 2009
Y1 - 2009
N2 - We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean and pea, Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic three developmental phases of legume seeds and the presence of embryo, endosperm and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined and this indicates that the composition of mature Lotus seed is more similar to soybean than pea. In a first attempt to determine the seed proteome both a 2-D PAGE approach and a GeLC-MS/MS approach were used. Globulins were analyzed by 2-D PAGE and five legumins, LLP1-5, and two convicilins, LCP1-2, were identified by MALDI Q-TOF mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a GeLC-MS/MS approach was used, and 665 and 181 unique proteins corresponding to gene accession numbers were identified for the two phases respectively. All the proteome data including the experimental data and MS spectra peaks were collected in a database that is available to the scientific community via a web-interface (http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi). This database establishes the basis for relating physiology, biochemistry and regulation of seed development in Lotus. Together with a new a web-interface (http://bioinfoserver.rsbs.anu.edu.au/utils/PathExpress4legumes/) collecting all protein identifications for Lotus, Medicago, and soybean seed proteomes, this database is a valuable resource for comparative seed proteomics and pathway analysis within and beyond the legume family.
AB - We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean and pea, Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic three developmental phases of legume seeds and the presence of embryo, endosperm and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined and this indicates that the composition of mature Lotus seed is more similar to soybean than pea. In a first attempt to determine the seed proteome both a 2-D PAGE approach and a GeLC-MS/MS approach were used. Globulins were analyzed by 2-D PAGE and five legumins, LLP1-5, and two convicilins, LCP1-2, were identified by MALDI Q-TOF mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a GeLC-MS/MS approach was used, and 665 and 181 unique proteins corresponding to gene accession numbers were identified for the two phases respectively. All the proteome data including the experimental data and MS spectra peaks were collected in a database that is available to the scientific community via a web-interface (http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi). This database establishes the basis for relating physiology, biochemistry and regulation of seed development in Lotus. Together with a new a web-interface (http://bioinfoserver.rsbs.anu.edu.au/utils/PathExpress4legumes/) collecting all protein identifications for Lotus, Medicago, and soybean seed proteomes, this database is a valuable resource for comparative seed proteomics and pathway analysis within and beyond the legume family.
U2 - 10.1104/pp.108.133405
DO - 10.1104/pp.108.133405
M3 - Journal article
C2 - 19129418
SN - 0032-0889
JO - Plant Physiology
JF - Plant Physiology
ER -