The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production

Manfred Schmid; Pawel Olszewski; Vicent Pelechano; Ishaan Gupta; Lars M Steinmetz; Torben Heick Jensen

doi:10.1016/j.celrep.2015.06.008

The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production

Manfred Schmid, Pawel Olszewski, Vicent Pelechano, Ishaan Gupta, Lars M Steinmetz, Torben Heick Jensen

Department of Molecular Biology and Genetics - RNA Biology and Innovation

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

45 Citations (Scopus)

Abstract

Polyadenylation of mRNA is a key step in eukaryotic gene expression. However, despite the major impact of poly(A) tails on mRNA metabolism, the precise roles of poly(A)-binding proteins (PABPs) in nuclear mRNA biogenesis remain elusive. Here, we demonstrate that rapid nuclear depletion of the S. cerevisiae PABP Nab2p leads to a global loss of cellular mRNA, but not of RNA lacking poly(A) tails. Disappearance of mRNA is a nuclear event, but not due to decreased transcription. Instead, the absence of Nab2p results in robust nuclear mRNA decay by the ribonucleolytic RNA exosome in a polyadenylation-dependent process. We conclude that Nab2p is required to protect early mRNA and therefore constitutes a crucial nuclear mRNA biogenesis factor.

Original language	English
Journal	Cell Reports
Volume	12
Issue	1
Pages (from-to)	128-139
Number of pages	12
ISSN	2211-1247
DOIs	https://doi.org/10.1016/j.celrep.2015.06.008
Publication status	Published - 7 Jul 2015

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http://www.sciencedirect.com/science/article/pii/S2211124715006063Licence: CC BY-NC-ND

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title = "The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production",

abstract = "Polyadenylation of mRNA is a key step in eukaryotic gene expression. However, despite the major impact of poly(A) tails on mRNA metabolism, the precise roles of poly(A)-binding proteins (PABPs) in nuclear mRNA biogenesis remain elusive. Here, we demonstrate that rapid nuclear depletion of the S. cerevisiae PABP Nab2p leads to a global loss of cellular mRNA, but not of RNA lacking poly(A) tails. Disappearance of mRNA is a nuclear event, but not due to decreased transcription. Instead, the absence of Nab2p results in robust nuclear mRNA decay by the ribonucleolytic RNA exosome in a polyadenylation-dependent process. We conclude that Nab2p is required to protect early mRNA and therefore constitutes a crucial nuclear mRNA biogenesis factor.",

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T1 - The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production

AU - Schmid, Manfred

AU - Olszewski, Pawel

AU - Pelechano, Vicent

AU - Gupta, Ishaan

AU - Steinmetz, Lars M

AU - Jensen, Torben Heick

PY - 2015/7/7

Y1 - 2015/7/7

N2 - Polyadenylation of mRNA is a key step in eukaryotic gene expression. However, despite the major impact of poly(A) tails on mRNA metabolism, the precise roles of poly(A)-binding proteins (PABPs) in nuclear mRNA biogenesis remain elusive. Here, we demonstrate that rapid nuclear depletion of the S. cerevisiae PABP Nab2p leads to a global loss of cellular mRNA, but not of RNA lacking poly(A) tails. Disappearance of mRNA is a nuclear event, but not due to decreased transcription. Instead, the absence of Nab2p results in robust nuclear mRNA decay by the ribonucleolytic RNA exosome in a polyadenylation-dependent process. We conclude that Nab2p is required to protect early mRNA and therefore constitutes a crucial nuclear mRNA biogenesis factor.

AB - Polyadenylation of mRNA is a key step in eukaryotic gene expression. However, despite the major impact of poly(A) tails on mRNA metabolism, the precise roles of poly(A)-binding proteins (PABPs) in nuclear mRNA biogenesis remain elusive. Here, we demonstrate that rapid nuclear depletion of the S. cerevisiae PABP Nab2p leads to a global loss of cellular mRNA, but not of RNA lacking poly(A) tails. Disappearance of mRNA is a nuclear event, but not due to decreased transcription. Instead, the absence of Nab2p results in robust nuclear mRNA decay by the ribonucleolytic RNA exosome in a polyadenylation-dependent process. We conclude that Nab2p is required to protect early mRNA and therefore constitutes a crucial nuclear mRNA biogenesis factor.

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DO - 10.1016/j.celrep.2015.06.008

M3 - Journal article

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VL - 12

SP - 128

EP - 139

JO - Cell Reports

JF - Cell Reports

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The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production

Abstract

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