TY - JOUR
T1 - The multitasking polyA tail
T2 - nuclear RNA maturation, degradation and export
AU - Tudek, Agnieszka
AU - Lloret-Llinares, Marta
AU - Jensen, Torben Heick
N1 - © 2018 The Author(s).
PY - 2018/12/19
Y1 - 2018/12/19
N2 - A polyA (pA) tail is an essential modification added to the 30 ends of a wide range of RNAs at different stages of their metabolism. Here, we describe the main sources of polyadenylation and outline their underlying biochemical interactions within the nuclei of budding yeast Saccharomyces cerevisiae, human cells and, when relevant, the fission yeast Schizosaccharomyces pombe. Polyadenylation mediated by the S. Cerevisiae Trf4/5 enzymes, and their human homologues PAPD5/7, typically leads to the 30-end trimming or complete decay of non-coding RNAs. By contrast, the primary function of canonical pA polymerases (PAPs) is to produce stable and nuclear export-competent mRNAs. However, this dichotomy is becoming increasingly blurred, at least in S. Pombe and human cells, where polyadenylation mediated by canonical PAPs may also result in transcript decay. This article is part of the theme issue '50 and 30 modifications controlling RNA degradation'.
AB - A polyA (pA) tail is an essential modification added to the 30 ends of a wide range of RNAs at different stages of their metabolism. Here, we describe the main sources of polyadenylation and outline their underlying biochemical interactions within the nuclei of budding yeast Saccharomyces cerevisiae, human cells and, when relevant, the fission yeast Schizosaccharomyces pombe. Polyadenylation mediated by the S. Cerevisiae Trf4/5 enzymes, and their human homologues PAPD5/7, typically leads to the 30-end trimming or complete decay of non-coding RNAs. By contrast, the primary function of canonical pA polymerases (PAPs) is to produce stable and nuclear export-competent mRNAs. However, this dichotomy is becoming increasingly blurred, at least in S. Pombe and human cells, where polyadenylation mediated by canonical PAPs may also result in transcript decay. This article is part of the theme issue '50 and 30 modifications controlling RNA degradation'.
KW - PolyA binding proteins
KW - RNA decay
KW - RNA export
KW - RNA polyadenylation
KW - TRAMP complex
KW - Transcription termination
UR - http://www.scopus.com/inward/record.url?scp=85056270385&partnerID=8YFLogxK
U2 - 10.1098/rstb.2018.0169
DO - 10.1098/rstb.2018.0169
M3 - Review
C2 - 30397105
SN - 0962-8436
VL - 373
JO - Philosophical Transactions of the Royal Society B: Biological Sciences
JF - Philosophical Transactions of the Royal Society B: Biological Sciences
IS - 1762
M1 - 20180169
ER -