The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer

Research output: Contribution to conferencePosterResearch

Standard

The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer. / Wiklund, Erik Digman.

2010. Poster session presented at Keystone Symposia - RNA Silencing: Mechanism, Biology and Application, Keystone, United States.

Research output: Contribution to conferencePosterResearch

Harvard

Wiklund, ED 2010, 'The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer', Keystone Symposia - RNA Silencing: Mechanism, Biology and Application, Keystone, United States, 14/01/2010 - 19/01/2010.

APA

Wiklund, E. D. (2010). The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer. Poster session presented at Keystone Symposia - RNA Silencing: Mechanism, Biology and Application, Keystone, United States.

CBE

Wiklund ED. 2010. The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer. Poster session presented at Keystone Symposia - RNA Silencing: Mechanism, Biology and Application, Keystone, United States.

MLA

Wiklund, Erik Digman The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer. <strong><strong>Keystone Symposia - RNA Silencing: Mechanism, Biology and Application</strong></strong>, 14 Jan 2010, Keystone, United States, Poster, 2010.

Vancouver

Wiklund ED. The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer. 2010. Poster session presented at Keystone Symposia - RNA Silencing: Mechanism, Biology and Application, Keystone, United States.

Author

Wiklund, Erik Digman. / The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer. Poster session presented at Keystone Symposia - RNA Silencing: Mechanism, Biology and Application, Keystone, United States.

Bibtex

@conference{2050f940735b11df8c1a000ea68e967b,
title = "The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer",
abstract = "MicroRNAs (miRNA) are small non-coding RNAs commonly deregulated in cancer. The miR-200 family (miR-200a, -200b, -200c, -141 and -429) and miR-205 are encoded in three separate genomic loci and are frequently silenced in advanced cancer. The miR-200 family and miR-205 have been implicated in tumour invasion and metastasis by negatively regulating the transcriptional repressors of E-cadherin, ZEB1 and ZEB2. Loss of miR-200 expression leads to silencing of E-cadherin, thereby promoting epithelial to mesenchymal transition (EMT) and loss of cell adhesion. However, little is known about the transcriptional regulation of the miR-200s. Here we show that the three miR-200 loci are concurrently silenced and gain promoter hypermethylation in muscle invasive bladder tumors and undifferentiated bladder cell lines. Furthermore, we report that the mesoderm transcription factor Twist1 and miR-200 expression is inversely correlated, and that Twist1 represses miR-200 expression in the normal bladder cell line HU609 by directly binding to E-box motifs in the miR-200 family and miR-205 promoters.  This is the first implication of a role for Twist1 in transcriptional repression of miRNAs and the first report demonstrating concerted transcriptional regulation of the three miR-200 loci. Our data also indicate that DNA hypermethylation of the miR-200 family and miR-205 are possible prognostic markers in bladder cancer.   ",
author = "Wiklund, {Erik Digman}",
year = "2010",
language = "English",
note = "null ; Conference date: 14-01-2010 Through 19-01-2010",

}

RIS

TY - CONF

T1 - The miR-200 family and miR-205 are repressed by Twist1 and concurrently silenced and DNA hypermethylated in invasive bladder cancer

AU - Wiklund, Erik Digman

N1 - Conference code: A7

PY - 2010

Y1 - 2010

N2 - MicroRNAs (miRNA) are small non-coding RNAs commonly deregulated in cancer. The miR-200 family (miR-200a, -200b, -200c, -141 and -429) and miR-205 are encoded in three separate genomic loci and are frequently silenced in advanced cancer. The miR-200 family and miR-205 have been implicated in tumour invasion and metastasis by negatively regulating the transcriptional repressors of E-cadherin, ZEB1 and ZEB2. Loss of miR-200 expression leads to silencing of E-cadherin, thereby promoting epithelial to mesenchymal transition (EMT) and loss of cell adhesion. However, little is known about the transcriptional regulation of the miR-200s. Here we show that the three miR-200 loci are concurrently silenced and gain promoter hypermethylation in muscle invasive bladder tumors and undifferentiated bladder cell lines. Furthermore, we report that the mesoderm transcription factor Twist1 and miR-200 expression is inversely correlated, and that Twist1 represses miR-200 expression in the normal bladder cell line HU609 by directly binding to E-box motifs in the miR-200 family and miR-205 promoters.  This is the first implication of a role for Twist1 in transcriptional repression of miRNAs and the first report demonstrating concerted transcriptional regulation of the three miR-200 loci. Our data also indicate that DNA hypermethylation of the miR-200 family and miR-205 are possible prognostic markers in bladder cancer.  

AB - MicroRNAs (miRNA) are small non-coding RNAs commonly deregulated in cancer. The miR-200 family (miR-200a, -200b, -200c, -141 and -429) and miR-205 are encoded in three separate genomic loci and are frequently silenced in advanced cancer. The miR-200 family and miR-205 have been implicated in tumour invasion and metastasis by negatively regulating the transcriptional repressors of E-cadherin, ZEB1 and ZEB2. Loss of miR-200 expression leads to silencing of E-cadherin, thereby promoting epithelial to mesenchymal transition (EMT) and loss of cell adhesion. However, little is known about the transcriptional regulation of the miR-200s. Here we show that the three miR-200 loci are concurrently silenced and gain promoter hypermethylation in muscle invasive bladder tumors and undifferentiated bladder cell lines. Furthermore, we report that the mesoderm transcription factor Twist1 and miR-200 expression is inversely correlated, and that Twist1 represses miR-200 expression in the normal bladder cell line HU609 by directly binding to E-box motifs in the miR-200 family and miR-205 promoters.  This is the first implication of a role for Twist1 in transcriptional repression of miRNAs and the first report demonstrating concerted transcriptional regulation of the three miR-200 loci. Our data also indicate that DNA hypermethylation of the miR-200 family and miR-205 are possible prognostic markers in bladder cancer.  

M3 - Poster

Y2 - 14 January 2010 through 19 January 2010

ER -