The importance of topoisomerases for chromatin regulated genes

Jacob Christian Fredsøe; Jakob Madsen Pedersen; Morten Terpager Rødgaard; Anni Hangaard Andersen

The importance of topoisomerases for chromatin regulated genes

Jacob Christian Fredsøe, Jakob Madsen Pedersen, Morten Terpager Rødgaard, Anni Hangaard Andersen

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Abstract

DNA topoisomerases are enzymes, which function to relieve torsional stress in the DNA helix by introducing transient breaks into the DNA molecule. By use of Saccharomyces cerevisiae and microarray technology we have previously shown that topoisomerases are required for the activation of chromatin regulated genes. A further study of the PHO5 gene belonging to this gene class demonstrated a lack of binding of the transcription factor Pho4 to the PHO5 promoter in the absence of topoisomerases, most likely due to changes in the overall superhelical state of the promoter.
The GAL genes also require topoisomerases for optimal activation, but in contrast to the PHO5 gene, topoisomerases are not required for chromatin remodeling of the GAL1/10 promoter region, indicating a different role of the enzymes. We are currently performing a detailed investigation of the GAL genes to elucidate the precise role of topoisomerases during the activation process, and the obtained results will be presented.

Original language	English
Publication date	26 Aug 2013
Number of pages	1
Publication status	Published - 26 Aug 2013

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Cite this

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title = "The importance of topoisomerases for chromatin regulated genes",

abstract = "DNA topoisomerases are enzymes, which function to relieve torsional stress in the DNA helix by introducing transient breaks into the DNA molecule. By use of Saccharomyces cerevisiae and microarray technology we have previously shown that topoisomerases are required for the activation of chromatin regulated genes. A further study of the PHO5 gene belonging to this gene class demonstrated a lack of binding of the transcription factor Pho4 to the PHO5 promoter in the absence of topoisomerases, most likely due to changes in the overall superhelical state of the promoter.The GAL genes also require topoisomerases for optimal activation, but in contrast to the PHO5 gene, topoisomerases are not required for chromatin remodeling of the GAL1/10 promoter region, indicating a different role of the enzymes. We are currently performing a detailed investigation of the GAL genes to elucidate the precise role of topoisomerases during the activation process, and the obtained results will be presented.",

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T1 - The importance of topoisomerases for chromatin regulated genes

AU - Fredsøe, Jacob Christian

AU - Pedersen, Jakob Madsen

AU - Rødgaard, Morten Terpager

AU - Andersen, Anni Hangaard

PY - 2013/8/26

Y1 - 2013/8/26

N2 - DNA topoisomerases are enzymes, which function to relieve torsional stress in the DNA helix by introducing transient breaks into the DNA molecule. By use of Saccharomyces cerevisiae and microarray technology we have previously shown that topoisomerases are required for the activation of chromatin regulated genes. A further study of the PHO5 gene belonging to this gene class demonstrated a lack of binding of the transcription factor Pho4 to the PHO5 promoter in the absence of topoisomerases, most likely due to changes in the overall superhelical state of the promoter.The GAL genes also require topoisomerases for optimal activation, but in contrast to the PHO5 gene, topoisomerases are not required for chromatin remodeling of the GAL1/10 promoter region, indicating a different role of the enzymes. We are currently performing a detailed investigation of the GAL genes to elucidate the precise role of topoisomerases during the activation process, and the obtained results will be presented.

AB - DNA topoisomerases are enzymes, which function to relieve torsional stress in the DNA helix by introducing transient breaks into the DNA molecule. By use of Saccharomyces cerevisiae and microarray technology we have previously shown that topoisomerases are required for the activation of chromatin regulated genes. A further study of the PHO5 gene belonging to this gene class demonstrated a lack of binding of the transcription factor Pho4 to the PHO5 promoter in the absence of topoisomerases, most likely due to changes in the overall superhelical state of the promoter.The GAL genes also require topoisomerases for optimal activation, but in contrast to the PHO5 gene, topoisomerases are not required for chromatin remodeling of the GAL1/10 promoter region, indicating a different role of the enzymes. We are currently performing a detailed investigation of the GAL genes to elucidate the precise role of topoisomerases during the activation process, and the obtained results will be presented.

M3 - Poster

ER -