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The human cap-binding complex is functionally connected to the nuclear RNA exosome

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The human cap-binding complex is functionally connected to the nuclear RNA exosome. / Andersen, Peter Refsing; Domanski, Michal; Kristiansen, Maiken S; Storvall, Helena; Ntini, Evgenia; Verheggen, Celine; Schein, Aleks; Bunkenborg, Jakob; Poser, Ina; Hallais, Marie; Sandberg, Rickard; Hyman, Anthony; Lacava, John; Rout, Michael P; Andersen, Jens Sandberg; Bertrand, Edouard; Jensen, Torben Heick.

In: Nature Structural and Molecular Biology, Vol. 20, No. 12, 24.11.2013, p. 1367-1376.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Andersen, PR, Domanski, M, Kristiansen, MS, Storvall, H, Ntini, E, Verheggen, C, Schein, A, Bunkenborg, J, Poser, I, Hallais, M, Sandberg, R, Hyman, A, Lacava, J, Rout, MP, Andersen, JS, Bertrand, E & Jensen, TH 2013, 'The human cap-binding complex is functionally connected to the nuclear RNA exosome', Nature Structural and Molecular Biology, vol. 20, no. 12, pp. 1367-1376. https://doi.org/10.1038/nsmb.2703

APA

Andersen, P. R., Domanski, M., Kristiansen, M. S., Storvall, H., Ntini, E., Verheggen, C., ... Jensen, T. H. (2013). The human cap-binding complex is functionally connected to the nuclear RNA exosome. Nature Structural and Molecular Biology, 20(12), 1367-1376. https://doi.org/10.1038/nsmb.2703

CBE

Andersen PR, Domanski M, Kristiansen MS, Storvall H, Ntini E, Verheggen C, Schein A, Bunkenborg J, Poser I, Hallais M, Sandberg R, Hyman A, Lacava J, Rout MP, Andersen JS, Bertrand E, Jensen TH. 2013. The human cap-binding complex is functionally connected to the nuclear RNA exosome. Nature Structural and Molecular Biology. 20(12):1367-1376. https://doi.org/10.1038/nsmb.2703

MLA

Vancouver

Andersen PR, Domanski M, Kristiansen MS, Storvall H, Ntini E, Verheggen C et al. The human cap-binding complex is functionally connected to the nuclear RNA exosome. Nature Structural and Molecular Biology. 2013 Nov 24;20(12):1367-1376. https://doi.org/10.1038/nsmb.2703

Author

Andersen, Peter Refsing ; Domanski, Michal ; Kristiansen, Maiken S ; Storvall, Helena ; Ntini, Evgenia ; Verheggen, Celine ; Schein, Aleks ; Bunkenborg, Jakob ; Poser, Ina ; Hallais, Marie ; Sandberg, Rickard ; Hyman, Anthony ; Lacava, John ; Rout, Michael P ; Andersen, Jens Sandberg ; Bertrand, Edouard ; Jensen, Torben Heick. / The human cap-binding complex is functionally connected to the nuclear RNA exosome. In: Nature Structural and Molecular Biology. 2013 ; Vol. 20, No. 12. pp. 1367-1376.

Bibtex

@article{dd24ec316ed145bea06cd22b14909fec,
title = "The human cap-binding complex is functionally connected to the nuclear RNA exosome",
abstract = "Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN). RNA immunoprecipitation using CBCN factors as well as the analysis of combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through products of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5' cap links transcription termination to exosomal RNA degradation through CBCN.",
author = "Andersen, {Peter Refsing} and Michal Domanski and Kristiansen, {Maiken S} and Helena Storvall and Evgenia Ntini and Celine Verheggen and Aleks Schein and Jakob Bunkenborg and Ina Poser and Marie Hallais and Rickard Sandberg and Anthony Hyman and John Lacava and Rout, {Michael P} and Andersen, {Jens Sandberg} and Edouard Bertrand and Jensen, {Torben Heick}",
year = "2013",
month = "11",
day = "24",
doi = "10.1038/nsmb.2703",
language = "English",
volume = "20",
pages = "1367--1376",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "Nature Publishing Group",
number = "12",

}

RIS

TY - JOUR

T1 - The human cap-binding complex is functionally connected to the nuclear RNA exosome

AU - Andersen, Peter Refsing

AU - Domanski, Michal

AU - Kristiansen, Maiken S

AU - Storvall, Helena

AU - Ntini, Evgenia

AU - Verheggen, Celine

AU - Schein, Aleks

AU - Bunkenborg, Jakob

AU - Poser, Ina

AU - Hallais, Marie

AU - Sandberg, Rickard

AU - Hyman, Anthony

AU - Lacava, John

AU - Rout, Michael P

AU - Andersen, Jens Sandberg

AU - Bertrand, Edouard

AU - Jensen, Torben Heick

PY - 2013/11/24

Y1 - 2013/11/24

N2 - Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN). RNA immunoprecipitation using CBCN factors as well as the analysis of combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through products of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5' cap links transcription termination to exosomal RNA degradation through CBCN.

AB - Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN). RNA immunoprecipitation using CBCN factors as well as the analysis of combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through products of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5' cap links transcription termination to exosomal RNA degradation through CBCN.

U2 - 10.1038/nsmb.2703

DO - 10.1038/nsmb.2703

M3 - Journal article

C2 - 24270879

VL - 20

SP - 1367

EP - 1376

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

SN - 1545-9993

IS - 12

ER -