The herpesviral antagonist m152 reveals differential activation of STING-dependent IRF and NF-κB signaling and STING's dual role during MCMV infection

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DOI

  • Markus Stempel, Helmholtz Centre for Infection Research (HZI)
  • ,
  • Baca Chan, Helmholtz Centre for Infection Research (HZI)
  • ,
  • Vanda Juranić Lisnić, University of Rijeka
  • ,
  • Astrid Krmpotić, University of Rijeka
  • ,
  • Josephine Hartung, Helmholtz Centre for Infection Research (HZI)
  • ,
  • Søren R. Paludan
  • Nadia Füllbrunn, Helmholtz Centre for Infection Research (HZI)
  • ,
  • Niels A.W. Lemmermann, Johannes Gutenberg-Universität Mainz
  • ,
  • Melanie M. Brinkmann, Helmholtz Centre for Infection Research (HZI), TU Braunschweig

Cytomegaloviruses (CMVs) are master manipulators of the host immune response. Here, we reveal that the murine CMV (MCMV) protein m152 specifically targets the type I interferon (IFN) response by binding to stimulator of interferon genes (STING), thereby delaying its trafficking to the Golgi compartment from where STING initiates type I IFN signaling. Infection with an MCMV lacking m152 induced elevated type I IFN responses and this leads to reduced viral transcript levels both in vitro and in vivo. This effect is ameliorated in the absence of STING. Interestingly, while m152 inhibits STING-mediated IRF signaling, it did not affect STING-mediated NF-κB signaling. Analysis of how m152 targets STING translocation reveals that STING activates NF-κB signaling already from the ER prior to its trafficking to the Golgi. Strikingly, this response is important to promote early MCMV replication. Our results show that MCMV has evolved a mechanism to specifically antagonize the STING-mediated antiviral IFN response, while preserving its pro-viral NF-κB response, providing an advantage in the establishment of an infection.

Original languageEnglish
Article numbere100983
JournalEMBO Journal
Volume38
Issue5
ISSN0261-4189
DOIs
Publication statusPublished - 1 Mar 2019

    Research areas

  • herpesvirus, innate immunity, IRF3, NF-κB, STING

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