The embryonic expression patterns of zebrafish genes encoding LysM-domains

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  • Fabrice Jean Francois Laroche, Denmark
  • C Tulotta, Leiden University, Institute of Biology, Molecular Cell Biology Department, Leiden University, Netherlands
  • G E M Lamers, Leiden University, Institute of Biology, Molecular Cell Biology Department, Leiden University, Netherlands
  • A H Meijer, Leiden University, Institute of Biology, Molecular Cell Biology Department, Leiden University, Netherlands
  • P Yang, Leiden Institute for Advanced Computer sciences (LIACS), Leiden University, Netherlands
  • F J Verbeek, Leiden Institute for Advanced Computer sciences (LIACS), Leiden University, Netherlands
  • Mickael Blaise, Denmark
  • Jens Stougaard
  • H P Spaink, Centre for Carbohydrate Recognition and Signalling, Department of Molecular Biology and Genetics, Aarhus University, Denmark
The function and structure of LysM-domain containing proteins are very diverse. Although some LysM domains are able to bind peptidoglycan or chitin type carbohydrates in bacteria, in fungi and in plants, the function(s) of vertebrate LysM domains and proteins remains largely unknown. In this study we have identified and annotated the six zebrafish genes of this family, which encode at least ten conceptual LysM-domain containing proteins. Two distinct sub-families called LysMD and OXR were identified and shown to be highly conserved across vertebrates. The detailed characterization of LysMD and OXR gene expression in zebrafish embryos showed that all the members of these sub-families are strongly expressed maternally and zygotically from the earliest stages of a vertebrate embryonic development. Moreover, the analysis of the spatio-temporal expression patterns, by whole mount and fluorescent in situ hybridizations, demonstrates pronounced LysMD and OXR gene expression in the zebrafish brain and nervous system during stages of larval development. None of the zebrafish LysMD or OXR genes was responsive to challenge with bacterial pathogens in embryo models of Salmonella and Mycobacterium infections. In addition, the expression patterns of the OXR genes were mapped in a zebrafish brain atlas.
Original languageEnglish
JournalGene Expression Patterns
Volume13
Issue7
Pages (from-to)212-224
Number of pages13
ISSN1567-133X
DOIs
Publication statusPublished - Oct 2013

    Research areas

  • Animals, Brain, Embryo, Nonmammalian, Embryonic Development, Gene Expression Regulation, Developmental, Humans, In Situ Hybridization, Fluorescence, Mycobacterium Infections, Nontuberculous, Phylogeny, Protein Interaction Domains and Motifs, Proteins, Salmonella Infections, Animal, Salmonella typhimurium, Sequence Alignment, Spatio-Temporal Analysis, Zebrafish, Zebrafish Proteins

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