The embryonic expression patterns of zebrafish genes encoding LysM-domains

TY - JOUR

T1 - The embryonic expression patterns of zebrafish genes encoding LysM-domains

AU - Laroche, Fabrice Jean Francois

AU - Tulotta, C

AU - Lamers, G E M

AU - Meijer, A H

AU - Yang, P

AU - Verbeek, F J

AU - Blaise, Mickael

AU - Stougaard, Jens

AU - Spaink, H P

N1 - Copyright © 2013 Elsevier B.V. All rights reserved.

PY - 2013/10

Y1 - 2013/10

N2 - The function and structure of LysM-domain containing proteins are very diverse. Although some LysM domains are able to bind peptidoglycan or chitin type carbohydrates in bacteria, in fungi and in plants, the function(s) of vertebrate LysM domains and proteins remains largely unknown. In this study we have identified and annotated the six zebrafish genes of this family, which encode at least ten conceptual LysM-domain containing proteins. Two distinct sub-families called LysMD and OXR were identified and shown to be highly conserved across vertebrates. The detailed characterization of LysMD and OXR gene expression in zebrafish embryos showed that all the members of these sub-families are strongly expressed maternally and zygotically from the earliest stages of a vertebrate embryonic development. Moreover, the analysis of the spatio-temporal expression patterns, by whole mount and fluorescent in situ hybridizations, demonstrates pronounced LysMD and OXR gene expression in the zebrafish brain and nervous system during stages of larval development. None of the zebrafish LysMD or OXR genes was responsive to challenge with bacterial pathogens in embryo models of Salmonella and Mycobacterium infections. In addition, the expression patterns of the OXR genes were mapped in a zebrafish brain atlas.

AB - The function and structure of LysM-domain containing proteins are very diverse. Although some LysM domains are able to bind peptidoglycan or chitin type carbohydrates in bacteria, in fungi and in plants, the function(s) of vertebrate LysM domains and proteins remains largely unknown. In this study we have identified and annotated the six zebrafish genes of this family, which encode at least ten conceptual LysM-domain containing proteins. Two distinct sub-families called LysMD and OXR were identified and shown to be highly conserved across vertebrates. The detailed characterization of LysMD and OXR gene expression in zebrafish embryos showed that all the members of these sub-families are strongly expressed maternally and zygotically from the earliest stages of a vertebrate embryonic development. Moreover, the analysis of the spatio-temporal expression patterns, by whole mount and fluorescent in situ hybridizations, demonstrates pronounced LysMD and OXR gene expression in the zebrafish brain and nervous system during stages of larval development. None of the zebrafish LysMD or OXR genes was responsive to challenge with bacterial pathogens in embryo models of Salmonella and Mycobacterium infections. In addition, the expression patterns of the OXR genes were mapped in a zebrafish brain atlas.

KW - Animals

KW - Brain

KW - Embryo, Nonmammalian

KW - Embryonic Development

KW - Gene Expression Regulation, Developmental

KW - Humans

KW - In Situ Hybridization, Fluorescence

KW - Mycobacterium Infections, Nontuberculous

KW - Phylogeny

KW - Protein Interaction Domains and Motifs

KW - Proteins

KW - Salmonella Infections, Animal

KW - Salmonella typhimurium

KW - Sequence Alignment

KW - Spatio-Temporal Analysis

KW - Zebrafish

KW - Zebrafish Proteins

U2 - 10.1016/j.gep.2013.02.007

DO - 10.1016/j.gep.2013.02.007

M3 - Journal article

C2 - 23567754

SN - 1567-133X

VL - 13

SP - 212

EP - 224

JO - Gene Expression Patterns

JF - Gene Expression Patterns

IS - 7

ER -