The effect of mannan-binding lectin on opsonophagocytosis of Neisseria meningitidis

M Drogari-Apiranthitou, C A Fijen, S Thiel, A Platonov, L Jensen, J Dankert, E J Kuijper

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

    Abstract

    Mannan-binding lectin (MBL), an acute phase protein with a structure and a function very similar to that of C1q, is known to act as an opsonin binding to a number of microorganisms. In order to investigate the effect of MBL on the phagocytic killing of meningococci, a serogroup B meningococcal strain (H44/76) and its unencapsulated variant v24, as well as a serogroup A meningococcal strain were opsonized with MBL (purified from normal human plasma at the State Serum Institute, Denmark) and used in a phagocytic killing assay at a density of 7 x 10(3) CFU/ml. Polymorphonuclear cells (PMNs) from one healthy donor were isolated by density gradient centrifugation over Percoll and added to the system (7 x 10(6) cells/ml). In a first set of experiments without addition of serum or complement, no influence of MBL was observed on the killing of any of these strains. Addition of MBL to non-opsonized bacteria of the serogroup A strain did not result in enhanced killing either; on the contrary, the growth of this strain increased significantly when a high MBL concentration (40 micrograms/ml) was used in the presence of PMNs. Further investigations were performed using sera of five individuals with late complement component deficiency (LCCD) and a concomitant MBL deficiency, vaccinated with a tetra-valent (ACYW135) meningococcal capsular polysaccharide vaccine. Pre- and post-vaccination sera (50% final concentration) were tested against a group A strain opsonized or not with MBL. In only one patient was there a moderate increase of killing of the opsonized bacteria after vaccination compared to pre-vaccination serum. Our results suggest that MBL may not play a significant role in the opsonophagocytosis of meningococci, irrespective of its binding to unencapsulated and serogroup A strains.
    Original languageEnglish
    JournalInternational Immunopharmacology
    Volume38
    Issue1-2
    Pages (from-to)93-9
    Number of pages7
    ISSN1567-5769
    Publication statusPublished - 1 Dec 1997

    Keywords

    • Bacterial Vaccines
    • Carrier Proteins
    • Collectins
    • Colony-Forming Units Assay
    • Complement C1q
    • Complement System Proteins
    • Humans
    • Lectins
    • Mannans
    • Neisseria meningitidis
    • Opsonin Proteins
    • Phagocytosis
    • Vaccination

    Fingerprint

    Dive into the research topics of 'The effect of mannan-binding lectin on opsonophagocytosis of Neisseria meningitidis'. Together they form a unique fingerprint.

    Cite this